School of Biotechnology, Shri Mata Vaishno Devi University, Katra, Jammu & Kashmir, 182320, India.
Instrumentation Division, CSIR - Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India.
Sci Rep. 2020 Feb 5;10(1):1926. doi: 10.1038/s41598-020-58676-2.
The nutritional challenge faced by the monogastric animals due to the chelation effects of phytic acid, fuel the research on bioprospecting of probiotics for phytase production. Pediococcus acidilactici SMVDUDB2 isolated from Kalarei, exhibited extracellular phytase activity of 5.583 U/mL after statistical optimization of fermentation conditions viz. peptone (1.27%); temperature (37 °C); pH (6.26) and maltose (1.43%). The phytase enzyme possessed optimum pH and temperature of 5.5 and 37 °C, respectively and was thermostable at 60 °C. The enzyme was purified 6.42 fold with a specific activity of 245.12 U/mg with hydrophobic interaction chromatography. The purified enzyme had K and V values of 0.385 mM and 4.965 μmol/min respectively, with sodium phytate as substrate. The strain depicted more than 80% survival rate at low pH (pH 2.0, 3.0), high bile salt concentration (0.3 and 0.5%), after gastrointestinal transit, highest hydrophobicity affinity with ethyl acetate (33.33 ± 0%), autoaggregation (77.68 ± 0.68%) as well as coaggregation (73.57 ± 0.47%) with Staphylococcus aureus (MTCC 3160). The strain exhibited antimicrobial activity against Bacillus subtilis (MTCC 121), Mycobacterium smegmatis (MTCC 994), Staphylococcus aureus (MTCC 3160), Proteus vulgaris (MTCC 426), Escherichia coli (MTCC 1652) and Lactobacillus rhamnosus (MTCC 1408). The amount of exopolysaccharide produced by the strain was 2 g/L. This strain having the capability of phytate degradation and possessing probiotic traits could find application in food and feed sectors.
由于植酸的螯合作用，单胃动物面临营养挑战，这推动了植酸酶生产的益生菌生物勘探研究。从卡拉雷分离出的乳酸片球菌 SMVDUDB2 表现出细胞外植酸酶活性为 5.583 U/mL，经过发酵条件的统计优化，即蛋白胨（1.27%）；温度（37°C）；pH（6.26）和麦芽糖（1.43%）。植酸酶的最适 pH 和温度分别为 5.5 和 37°C，在 60°C 下具有热稳定性。该酶通过疏水相互作用色谱法纯化 6.42 倍，比活为 245.12 U/mg。纯化酶以植酸钠为底物，具有 0.385 mM 的 K 和 4.965 μmol/min 的 V 值。该菌株在低 pH（pH 2.0、3.0）、高胆汁盐浓度（0.3%和 0.5%）下表现出超过 80%的存活率，在胃肠道转运后，与乙基乙酸酯（33.33±0%）具有最高的疏水性亲和力，自动聚集（77.68±0.68%）以及与金黄色葡萄球菌（MTCC 3160）的共聚集（73.57±0.47%）。该菌株对枯草芽孢杆菌（MTCC 121）、耻垢分枝杆菌（MTCC 994）、金黄色葡萄球菌（MTCC 3160）、普通变形杆菌（MTCC 426）、大肠杆菌（MTCC 1652）和鼠李糖乳杆菌（MTCC 1408）具有抗菌活性。该菌株产生的胞外多糖量为 2 g/L。该菌株具有植酸降解能力和益生菌特性，可在食品和饲料领域得到应用。
