Shrivastava Swati, Sharma Nidhi, Sharma Abhishek, Gupta Shubhi
Department of Biochemistry, Government Medical College, Datia, Madhya Pradesh, India.
Department of Microbiology, Government Medical College, Datia, Madhya Pradesh, India.
Bioinformation. 2025 May 31;21(5):1163-1168. doi: 10.6026/973206300211163. eCollection 2025.
Probiotic strains, defined as live microorganisms that confer health benefits to the host when administered in adequate amounts, are widely incorporated into commercial products. Therefore, assessing the safety and functional attributes of 20 commercially available probiotic strains using complete genome sequencing is of immediate importance. Our analysis revealed that 15% of the products were mislabeled, containing species different from those declared on the label. Further, 12% of the tested strains harbored transferable antimicrobial resistance genes. However, 8% Bacillus species contained virulence-associated genes. Nonetheless probiotic-related genes linked to acid and bile tolerance were consistently found in Lactobacillus strains. These findings underscore the urgent need for standardized genomic safety assessments prior to the market approval of probiotic products.
益生菌菌株被定义为当适量施用时能给宿主带来健康益处的活微生物，已被广泛应用于商业产品中。因此，利用全基因组测序评估20种市售益生菌菌株的安全性和功能特性具有迫切的重要性。我们的分析表明，15%的产品存在标签错误，所含菌种与标签上宣称的不同。此外，12%的测试菌株含有可转移的抗菌抗性基因。然而，8%的芽孢杆菌属菌种含有与毒力相关的基因。尽管如此，在乳酸杆菌属菌株中始终能发现与耐酸和耐胆汁相关的益生菌相关基因。这些发现凸显了在益生菌产品上市批准前进行标准化基因组安全评估的迫切需求。
