Trivedi Roopsi, Sangur Rajashekar, Bathala Lakshmana Rao, Srivastava Shitij, Madhav Srishti, Chaubey Preeti
Department of Prosthodontics, Sardar Patel Postgraduate Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India.
Department of Prosthodontics, Rama Dental College Hospital and Research Centre, Kanpur, Uttar Pradesh, India.
J Med Life. 2019 Oct-Dec;12(4):395-402. doi: 10.25122/jml-2019-0050.
The need to use a natural alternative for disinfecting dental impression materials, which should be biocompatible and effective, led us to evaluate the efficacy of Aloe vera as a disinfectant by immersion and spray method on alginate impression material and its effect on the dimensional stability of the resultant gypsum cast. The efficacy of the disinfection procedures against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans was evaluated by determining the number of colony-forming units (CFU) recovered after disinfection of alginate discs inoculated with 1x106 CFU for defined intervals with aloe vera solution (99.96%). Dimensional stability was determined from the mean percentage deviation of three measurements that were taken between the fixed points on the casts using a traveling microscope and compared with corresponding measurements from the master model and controls. Statistical analysis of data was determined by analysis of variance. We found out that there is a mean percentage reduction in colony count of S. aureus, P. aeruginosa, and C. albicans after 3 minutes of immersion in aloe vera and after 3 minutes spray disinfection. Complete elimination of all the microorganism cells after 7 min immersion and spray disinfection. There was a statistically significant difference in the increase of the mean anteroposterior (AP) and cross arch (CA) dimension after 3 and 7 minutes immersion in aloe vera. Spraying with aloe vera for 7 minutes was proved to be the most effective disinfection procedure without altering dimensional stability.
需要使用一种天然替代品对牙科印模材料进行消毒，这种替代品应具有生物相容性且有效，这促使我们通过浸泡和喷雾法评估芦荟作为消毒剂对藻酸盐印模材料的有效性及其对所得石膏模型尺寸稳定性的影响。通过测定用芦荟溶液（99.96%）对接种了1×10⁶CFU的藻酸盐圆盘进行规定时间消毒后回收的菌落形成单位（CFU）数量，评估消毒程序对金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌的有效性。尺寸稳定性通过使用移动显微镜在模型上的固定点之间进行的三次测量的平均百分比偏差来确定，并与主模型和对照的相应测量值进行比较。数据的统计分析通过方差分析来确定。我们发现，在芦荟中浸泡3分钟和喷雾消毒3分钟后，金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌的菌落计数平均百分比降低。在浸泡和喷雾消毒7分钟后，所有微生物细胞被完全消除。在芦荟中浸泡3分钟和7分钟后，平均前后径（AP）和跨弓径（CA）尺寸增加存在统计学显著差异。事实证明，喷雾芦荟7分钟是最有效的消毒程序，且不会改变尺寸稳定性。
