Veronesi Francesca, Vandenbulcke Filippo, Ashmore Kevin, Di Matteo Berardo, Nicoli Aldini Nicolò, Martini Lucia, Fini Milena, Kon Elizaveta
Laboratory of Preclinical and Surgical Studies, IRCCS Istituto Ortopedico Rizzoli, Via Di Barbiano 1/10, 40136, Bologna, Italy.
Department of Biomedical Sciences, Humanitas University, Via Rita Levi Montalcini 4, 20090, Pieve Emanuele - Milan, Italy.
Int Orthop. 2020 Apr;44(4):779-793. doi: 10.1007/s00264-020-04493-1. Epub 2020 Feb 5.
One of the major risk factors for OA is meniscectomy (Mx) that causes a rapid and progressive OA. Mx has been employed in various animal models, especially in large ones, to study preclinical safety and strategy effectiveness to counteract OA. The aim of the present study is to review in vivo studies, performed in sheep and published in the last ten years.
The search strategy was performed in three websites: www.scopus.com, www.pubmed.com, and www.webofknowledge.com, using "Meniscectomy and osteoarthritis in sheep" keywords.
The 25 included studies performed unilateral total medial Mx (MMx), unilateral partial MMx, bilateral MMx, unilateral total lateral Mx (LMx), unilateral partial LMx, and bilateral LMx and MMx combined with anterior cruciate ligament transaction. The most frequently performed is the unilateral total MMx that increases changes in cartilage and subchondral bone more than the other techniques. Gross evaluations, histology, radiography, and biochemical tests are used to assess the degree of OA. The most widely tested treatments are related to scaffolds with or without mesenchymal stem cells.
OA therapeutic strategies require the use of large animal models due to similarities with human joint anatomy. A protocol for future in vivo studies on post-traumatic OA is clarified.
半月板切除术（Mx）是骨关节炎（OA）的主要危险因素之一，可导致快速进展性OA。Mx已被应用于各种动物模型，尤其是大型动物模型，以研究临床前安全性和对抗OA的策略有效性。本研究的目的是回顾过去十年发表的在绵羊身上进行的体内研究。
在三个网站（www.scopus.com、www.pubmed.com和www.webofknowledge.com）上进行检索，使用“绵羊半月板切除术与骨关节炎”关键词。
纳入的25项研究进行了单侧全内侧半月板切除术（MMx）、单侧部分MMx、双侧MMx、单侧全外侧半月板切除术（LMx）、单侧部分LMx以及双侧LMx和MMx联合前交叉韧带切断术。最常进行的是单侧全MMx，与其他技术相比，它会增加软骨和软骨下骨的变化。采用大体评估、组织学、放射学和生化测试来评估OA的程度。测试最广泛的治疗方法与含或不含间充质干细胞的支架有关。
由于与人类关节解剖结构相似，OA治疗策略需要使用大型动物模型。明确了未来创伤后OA体内研究的方案。
