Veronesi Francesca, Fini Milena, Martini Lucia, Berardinelli Paolo, Russo Valentina, Filardo Giuseppe, Di Matteo Berardo, Marcacci Maurilio, Kon Elizaveta
Complex Structure of Surgical Sciences and Technologies, IRCCS Istituto Ortopedico Rizzoli, Via Di Barbiano 1/10, 40136 Bologna, Italy.
Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, Via Balzarini 1, 64100 Teramo, Italy.
Biology (Basel). 2022 Apr 28;11(5):681. doi: 10.3390/biology11050681.
The challenge of osteoarthritis (OA) is to find a minimally invasive orthobiological therapy to contrast OA progression, on inflammatory and structural fronts. The aim of the present study is to compare the effects of an intra-articular injection of three orthobiological treatments, autologous culture expanded adipose-derived mesenchymal stromal cells (ADSCs), autologous stromal vascular fraction (SVF) and allogenic culture expanded amniotic epithelial stem cells (AECs), in an animal model of OA. OA was induced in 24 sheep by bilateral lateral meniscectomy and, at 3 and 6 months post-treatment, the results were analyzed with macroscopy, histology, histomorphometry, and biochemistry. All the three treatments showed better results than control (injection of NaCl), but SVF and AECs showed superiority over ADSCs, because they induced higher cartilage regeneration and lower inflammation. SVF showed better results than AECs at 3 and 6 months. To conclude, SVF seems to be more favorable than the other biological options, because it is easily obtained and rapidly used after harvesting, with good healing potential. AECs cause no discomfort and could be also considered for the treatment of OA joints.
骨关节炎（OA）面临的挑战是找到一种微创的 orthobiological 疗法，从炎症和结构方面对抗 OA 的进展。本研究的目的是比较关节内注射三种 orthobiological 治疗方法，即自体培养扩增的脂肪来源间充质基质细胞（ADSCs）、自体基质血管成分（SVF）和异体培养扩增的羊膜上皮干细胞（AECs），在 OA 动物模型中的效果。通过双侧外侧半月板切除术在 24 只绵羊中诱导 OA，并在治疗后 3 个月和 6 个月，用宏观检查、组织学、组织形态计量学和生物化学方法分析结果。所有这三种治疗方法都比对照组（注射氯化钠）显示出更好的结果，但 SVF 和 AECs 比 ADSCs 显示出优势，因为它们诱导了更高的软骨再生和更低的炎症。在 3 个月和 6 个月时，SVF 比 AECs 显示出更好的结果。总之，SVF 似乎比其他生物选择更有利，因为它易于获取且收获后能迅速使用，具有良好的愈合潜力。AECs 不会引起不适，也可考虑用于 OA 关节的治疗。
