Department of Medicine, Federal University of São Carlos (UFSCar), São Carlos, Brazil.
Department of Physical Therapy, Federal University of São Carlos, São Carlos, Brazil.
Int J Exp Pathol. 2019 Oct;100(5-6):359-368. doi: 10.1111/iep.12340. Epub 2020 Feb 6.
The present study aimed to evaluate the effects of mesenchymal stromal cell (MSC) transplantation on motor function and collagen organization in the muscles of rats with type 1 diabetes mellitus. Male Wistar rats were randomly assigned to three groups: control (C), diabetic (DM) and diabetic treated with MSCs (DM-MSCs). Diabetes was induced by streptozotocin (50 µg/kg). Bone marrow cells were isolated from the tibia and femur. After 10 weeks of DM induction, the DM-MSC rats received four i.p. injections of MSCs (1 × 106). Ten weeks after MSC transplantation, motor performance was evaluated by the rotarod test and the anterior tibial (TA) muscles were collected for morphometric and quantification of collagen birefringence by polarizing microscopy analysis. Motor performance of the DM group was significantly reduced when compared to the C group and increased significantly in the DM + MSC group. The TA muscle mass was significantly reduced in the DM and DM + MSC groups compared to the C group. The connective tissue increased in the DM group compared to the C group and decreased in the DM + MSC group. The percentage collagen birefringence decreased significantly in the DM group when compared to the C group and increased in the DM + MSC group. Motor performance was positively correlated with collagen birefringence and negatively correlated with percentage of connective tissue. The results indicate that MSC transplantation improves both motor function and the collagen macromolecular organization in type 1 DM.
本研究旨在评估间充质干细胞（MSC）移植对 1 型糖尿病大鼠运动功能和肌肉胶原组织的影响。雄性 Wistar 大鼠随机分为三组：对照组（C）、糖尿病组（DM）和糖尿病 MSC 治疗组（DM-MSCs）。糖尿病通过链脲佐菌素（50 µg/kg）诱导。从胫骨和股骨中分离骨髓细胞。在 DM 诱导 10 周后，DM-MSC 大鼠接受四次腹腔注射 MSC（1×106）。MSC 移植 10 周后，通过转棒试验评估运动性能，收集前胫骨（TA）肌肉进行形态计量学和偏光显微镜分析胶原双折射的定量分析。与 C 组相比，DM 组的运动性能明显降低，而 DM+MSC 组的运动性能明显增加。与 C 组相比，DM 和 DM+MSC 组的 TA 肌肉质量明显减少。与 C 组相比，DM 组的结缔组织增加，而 DM+MSC 组的结缔组织减少。与 C 组相比，DM 组的胶原双折射百分比明显降低，而 DM+MSC 组的胶原双折射百分比增加。运动性能与胶原双折射呈正相关，与结缔组织百分比呈负相关。结果表明，MSC 移植可改善 1 型糖尿病大鼠的运动功能和胶原大分子组织。
