Brassolatti Patricia, Bossini Paulo Sérgio, Kido Hueliton Wilian, Derencio Oliveira Maria Carolina, Almeida-Lopes Luciana, Zanardi Lisinéia Maria, Napolitano Marcos Aurélio, Retto da Silva de Avó Lucimar, Araújo-Moreira Fernando M, Parizotto Nivaldo Antonio
Department of Morphology and Pathology, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil; Research and Education Center for Photo Therapy in Health Science (NUPEN), DMC Equipment Import and Export-Co. Ltda, São Carlos, SP, Brazil.
Research and Education Center for Photo Therapy in Health Science (NUPEN), DMC Equipment Import and Export-Co. Ltda, São Carlos, SP, Brazil.
J Tissue Viability. 2018 Nov;27(4):249-256. doi: 10.1016/j.jtv.2018.10.001. Epub 2018 Oct 4.
Burns are injuries caused mainly by thermal trauma, which can progress to unsatisfactory results healing. This study aimed to evaluate the biomaterial (bacterial cellulose membrane) and photobiomodulation, exclusively and associated, in the treatment of third degree burns in rats. Forty male Wistar rats (±280 g) were randomly divided into four groups, with 10 animals each: control group (CG); bacterial cellulose membrane group (MG); laser group (LG) and bacterial cellulose membrane and laser group (MG + L). The burn was caused with a 1 cm aluminum plate heated to 150 °C and pressed on the animal's back for 10 s. The treatments were started immediately after induction of injury. For to laser irradiation (660 nm, 100 mW, 25 J/cm and energy of 1 J) on five distinct application points were used, on alternate days, a total of five sessions. After ten days of treatment the animals were euthanized for collected samples. One-way ANOVA and Tukey's tests (P < 0.05) were used. Histological analysis revealed differences regarding the healing process phase in each experimental group. MG showed the proliferative phase. The LG demonstrated greater amount of blood vessels and immune expression of VEGF. However, when the treatments were combined, the number of vessels and the immune expression of VEGF factor was lower than LG. Thus, it was concluded that both treatments proposed (biomaterial and LLLT) are good alternatives for third degree burns when applied isolated because they stimulate the healing process by acting on the modulation of the inflammatory phase and promote stimulation of angiogenesis.
烧伤主要由热创伤引起，可能会导致愈合结果不理想。本研究旨在评估生物材料（细菌纤维素膜）和光生物调节单独及联合应用于大鼠三度烧伤治疗的效果。40只雄性Wistar大鼠（±280克）随机分为四组，每组10只动物：对照组（CG）；细菌纤维素膜组（MG）；激光组（LG）和细菌纤维素膜与激光联合组（MG + L）。使用一块加热至150°C的1厘米铝板按压动物背部10秒造成烧伤。损伤诱导后立即开始治疗。使用激光照射（660纳米，100毫瓦，25焦耳/平方厘米，能量1焦耳），在五个不同的照射点隔天进行，共五次照射。治疗十天后对动物实施安乐死以采集样本。采用单因素方差分析和Tukey检验（P < 0.05）。组织学分析显示各实验组在愈合过程阶段存在差异。MG组显示为增殖期。LG组显示血管数量更多且VEGF有免疫表达。然而，当两种治疗方法联合使用时，血管数量和VEGF因子的免疫表达低于LG组。因此得出结论，所提出的两种治疗方法（生物材料和低强度激光疗法）单独应用时都是三度烧伤的良好替代方案，因为它们通过作用于炎症阶段的调节来刺激愈合过程并促进血管生成。
