Ma Y, Zeng R, Hu Q-Q, Yan H-X, Yang L-X, Dong Y, Qu Y
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
College of Pharmacy, Southwest Minzu University, Chengdu, China.
Climacteric. 2020 Jun;23(3):279-287. doi: 10.1080/13697137.2020.1717462. Epub 2020 Feb 6.
The aim of the present study was to evaluate the effects of ethyl acetate extract of L. (POE) on ameliorating postmenopausal osteoporosis in ovariectomized (OVX) rats. Six-month-old female rats were randomly divided into seven groups: sham-operated; OVX; OVX with estradiol valerate; OVX with alendronate; and OVX with POE in graded doses (3.75, 5.0, or 7.5 g/kg/day). Administration began at week 6 after ovariectomy for 12 weeks. A comprehensive assessment of bone quality was performed, including serum biochemical markers, serum inflammatory factors, bone oxidative stress markers, bone mechanics, and bone histomorphometry. POE treatment significantly decelerated OVX-induced body weight gain without affecting the uterus index and produced a significant decrease in the levels of serum bone turnover markers ( < 0.05 or < 0.01). Biomechanical testing demonstrated that POE (5.0 and 7.5 g/kg/day) treatments significantly prevented the reduction in maximum stress and Young's modulus in OVX rats ( < 0.05). Compared with the OVX group, POE (3.75, 5.0, or 7.5 g/kg/day) treatments significantly increased trabecular bone mineral density by 35.03, 38.42, and 42.02%, respectively. Our findings suggest that POE has potential effects in regulation of bone metabolism and prevention of bone loss in postmenopausal osteoporosis.
本研究的目的是评估光果甘草乙酸乙酯提取物（POE）对改善去卵巢（OVX）大鼠绝经后骨质疏松症的作用。将6个月大的雌性大鼠随机分为7组：假手术组；去卵巢组；去卵巢加戊酸雌二醇组；去卵巢加阿仑膦酸钠组；以及去卵巢加不同剂量POE组（3.75、5.0或7.5 g/kg/天）。给药在卵巢切除术后第6周开始，持续12周。对骨质量进行了全面评估，包括血清生化标志物、血清炎症因子、骨氧化应激标志物、骨力学和骨组织形态计量学。POE治疗显著减缓了去卵巢诱导的体重增加，且不影响子宫指数，并使血清骨转换标志物水平显著降低（<0.05或<0.01）。生物力学测试表明，POE（5.0和7.5 g/kg/天）治疗显著防止了去卵巢大鼠最大应力和杨氏模量的降低（<0.05）。与去卵巢组相比，POE（3.75、5.0或7.5 g/kg/天）治疗分别使小梁骨矿物质密度显著增加了35.03%、38.42%和42.02%。我们的研究结果表明，POE在调节绝经后骨质疏松症的骨代谢和预防骨质流失方面具有潜在作用。
