Department of Botany, Islamia College Peshawar, Peshawar, Pakistan.
Department of Botany, University of Peshawar, Peshawar, Pakistan.
Microsc Res Tech. 2020 Jun;83(6):636-646. doi: 10.1002/jemt.23454. Epub 2020 Feb 6.
The present study was conducted on characterization of morpho-anatomical, phytochemical, and bio-elemental analysis of root, stem, and leaf of Verbascum thapsus. Morphologically Verbascum is a biennial plant that flowers for a month and a half in mid- to late summer. Various organoleptic features of root, leaf, and stem were recorded. Anatomically the T. S of the root, stem, and leaf showed a typical dicot histological differentiation. Leaf possessed anomocytic stomata, crescent shape vascular bundles, and covered with long and stellate type trichomes while, stem contained collateral type of vascular bundles and a well-developed pith to store phytochemicals responsible for various pharmacological activities. The powder drug study through scanning electron microscopy revealed the presence of various types of tissues. Branched, tree like and stellate trichomes in root and leaf help in absorption and reduce loss of water. These anatomical features are responsible for the survival of the plant as biennial. Four macro elements (Na, K, Ca, and Mg) and seven microelements (Cr, Cu, Fe, Mn, Ni, Zn, and Cd) and their concentrations in ppm were also studied using Atomic Absorption Spectroscopy. Phytochemical screening of methanolic extract showed existence of various secondary metabolites, while mucilage and anthraquinones was not detected. The present study helps to understand the taxonomic identification of the plant based on morpho-anatomical features and throws the attention of the researchers to carry out the work for developing its various formulations, which can ultimately be beneficial for the human beings as well as animals.
本研究对黄花烟草的根、茎和叶的形态解剖学、植物化学和生物元素分析进行了表征。形态上，黄花烟草是一种两年生植物，在夏末初秋开花一个半月。记录了根、叶和茎的各种感官特征。根、茎和叶的横切面显示出典型的双子叶组织分化。叶片具有不等细胞型气孔，新月形维管束，被长而星状的毛覆盖，而茎则含有侧生维管束和一个发达的髓来储存负责各种药理活性的植物化学物质。粉末药物通过扫描电子显微镜研究表明存在各种类型的组织。根和叶中的分枝状、树状和星状毛有助于吸收和减少水分流失。这些解剖学特征是该植物作为两年生植物生存的原因。还使用原子吸收光谱法研究了四种宏量元素（Na、K、Ca 和 Mg）和七种微量元素（Cr、Cu、Fe、Mn、Ni、Zn 和 Cd）及其在 ppm 中的浓度。甲醇提取物的植物化学筛选显示存在各种次生代谢物，而未检测到黏液质和蒽醌。本研究有助于根据形态解剖学特征对植物进行分类鉴定，并引起研究人员注意开展其各种制剂的研究，这最终将有益于人类和动物。
