Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Haizhou 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, 59 Cangwu Road, Haizhou 222005, China; Jiangsu Key Laboratory of Marine Biotechnology, 59 Cangwu Road, Haizhou 222005, China.
Int J Biol Macromol. 2020 Apr 15;149:877-881. doi: 10.1016/j.ijbiomac.2020.02.005. Epub 2020 Feb 3.
This study aimed to explore the effects of Astragalus membranaceus polysaccharide (AMP) on the growth and innate immunity of crucian carp (Carassius auratus). Crucian carps were randomly divided into a control group (fed with basal diet) and three AMP groups (received basal diet supplemented with 50, 100 and 150 mg/kg AMP). After 60 days of culture, the crucian carps from each group were weighed, and their immune indexes were measured. Another batch crucian carps from each group was injected with 0.15 ml of 10 CFU/ml Aeromonas hydrophila. The body weight gain, feed conversion rate, specific growth rate and digestive enzyme activity of the crucian carps in the low and middle doses of AMP groups were higher than those in the control group. The AMP groups had significantly higher survival rate and alkaline phosphatase level but lower glutamic-oxaloacetic transaminase, glutamic-alanine transaminase and serum bacteria number compared with the control group. The optimal dose of dietary AMP required for the maximum growth of crucian carp was 100 mg/kg. These results showed that AMP could promote the growth of crucian carps, improve their disease resistance and thus may be developed as a dietary supplement.
本研究旨在探讨黄芪多糖（AMP）对鲫鱼（Carassius auratus）生长和先天免疫的影响。鲫鱼被随机分为对照组（喂食基础饲料）和三个 AMP 组（喂食基础饲料，分别补充 50、100 和 150mg/kg AMP）。培养 60 天后，测量每组鲫鱼的体重和免疫指标。每组的另一批鲫鱼被注射 0.15ml 浓度为 10 CFU/ml 的嗜水气单胞菌。低、中剂量 AMP 组鲫鱼的体重增长率、饲料转化率、特定生长率和消化酶活性均高于对照组。与对照组相比，AMP 组鲫鱼的存活率和碱性磷酸酶水平显著升高，而谷氨酸草酰乙酸转氨酶、谷氨酸丙氨酸转氨酶和血清细菌数量显著降低。鲫鱼生长的最佳 AMP 饲料添加剂量为 100mg/kg。这些结果表明，AMP 可以促进鲫鱼的生长，提高其抗病能力，因此可能被开发为一种膳食补充剂。
