West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, PR China; School of Laboratory Medicine, Chengdu Medical College, Chengdu, 610500, PR China.
School of Food and Bioengineering, Xihua University, Chengdu, 610039, PR China.
Toxicology. 2020 Mar 30;433-434:152395. doi: 10.1016/j.tox.2020.152395. Epub 2020 Feb 3.
Copper, an essential microelement, can still be harmful to health and has a significant impact on the gut microbiota, which is closely related to health when copper is ingested excessively. However, the effects of low dose exposure to copper early in life on health and the gut microbiota are not well understood. Here, the effects of early-life exposure of copper on the toxicity, gut microbiota and the metabolome were investigated in Sprague-Dawley (SD) rats. The results showed that 0.20 and 1.00 mg/kg BW copper early-life exposure in SD rats significantly increased ALT, AST, and ALP levels in the blood and caused liver damage. Copper exposure had a dose-dependent effect on the alpha and beta diversity and reduced the abundance of probiotics, the ratio of Firmicutes to Bacteroidetes (F/B), and changed the abundance of fat metabolism and intestinal inflammation-related bacteria. The results of the fecal metabolome also demonstrated the effects of early-life copper exposure on liver damage and intestinal inflammation-related metabolic pathways. Together, our findings demonstrated that copper exposure during early life induced liver damage and gut microbiota dysbiosis and affected the relevant metabolic pathways.
铜是一种必需的微量元素，但摄入过量仍可能对健康造成危害，并对与健康密切相关的肠道微生物群产生重大影响。然而，人们对生命早期低剂量铜暴露对健康和肠道微生物群的影响还知之甚少。在这里，研究人员调查了生命早期暴露于铜对 Sprague-Dawley（SD）大鼠的毒性、肠道微生物群和代谢组的影响。结果表明，SD 大鼠生命早期暴露于 0.20 和 1.00mg/kg BW 的铜显著增加了血液中的 ALT、AST 和 ALP 水平，并导致肝脏损伤。铜暴露对 alpha 和 beta 多样性具有剂量依赖性影响，降低了益生菌的丰度、厚壁菌门与拟杆菌门的比例（F/B），并改变了脂肪代谢和肠道炎症相关细菌的丰度。粪便代谢组学的结果也证明了生命早期铜暴露对肝脏损伤和肠道炎症相关代谢途径的影响。总之，这些发现表明，生命早期的铜暴露会导致肝脏损伤和肠道微生物群失调，并影响相关的代谢途径。
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