卵泡液中的细胞因子谱在卵巢衰老过程中发生变化。

The cytokine profile of follicular fluid changes during ovarian ageing.

机构信息

Department of Reproductive Medicine, Angers University Hospital, 49000, Angers, France; MITOVASC Institute, CNRS 6015, INSERM U1083, Angers University, 49000, Angers, France.

Reproductive Biology Unit, Angers University Hospital, 49000, Angers, France.

出版信息

J Gynecol Obstet Hum Reprod. 2020 Apr;49(4):101704. doi: 10.1016/j.jogoh.2020.101704. Epub 2020 Feb 3.

DOI:10.1016/j.jogoh.2020.101704

PMID:32028036

Abstract

OBJECTIVE

Ovarian ageing is one of the commonest causes of infertility in patients consulting for assisted reproductive technology. The composition of the follicular fluid (FF), which reflects the exchanges between the oocyte and its microenvironment, has been extensively investigated to determine the metabolic pathways involved in various ovarian disorders. Considering the importance of cytokines in folliculogenesis, we focused on the cytokine profile of the FF during ovarian ageing.

MATERIAL AND METHODS

Our cross-sectional study assesses the levels of 27 cytokines and growth factors in the FF of two groups of women undergoing in vitro fertilization. One group included 28 patients with ovarian ageing clinically characterized by a diminished ovarian reserve (DOR), and the other group included 29 patients with a normal ovarian reserve (NOR), serving as controls.

RESULTS

With univariate analysis, the cytokine profile was found to differ significantly between the two groups. After adjustment of the p-values, platelet-derived growth factor-BB (PDGF-BB) was the only cytokine with a significantly lower concentration in the DOR group (7.34 ± 16.11 pg/mL) than in the NOR group (24.39 ± 41.38 pg/mL) (p = 0.005), independently of chronological age.

CONCLUSION

Thus, PDGF-BB would seem to be implicated in the physiopathology of DOR, potentially in relation to its role in folliculogenesis or in the protection against oxidative stress.

摘要

目的

卵巢衰老（aging）是患者在寻求辅助生殖技术（assisted reproductive technology）时出现不孕（infertility）的最常见原因之一。卵泡液（follicular fluid，FF）的组成反映了卵母细胞与其微环境之间的物质交换，其代谢途径已广泛研究，以确定各种卵巢疾病的代谢途径。鉴于细胞因子（cytokines）在卵泡发生（folliculogenesis）中的重要性，我们专注于卵巢衰老时 FF 中的细胞因子谱（profile）。

材料和方法

我们的横断面研究评估了接受体外受精（in vitro fertilization）的两组女性 FF 中 27 种细胞因子和生长因子的水平。一组包括 28 例卵巢储备功能降低（diminished ovarian reserve，DOR）的临床特征为卵巢衰老的患者，另一组包括 29 例具有正常卵巢储备（normal ovarian reserve，NOR）的患者作为对照组。

结果

单变量分析发现，两组之间的细胞因子谱存在显著差异。在调整 p 值后，血小板衍生生长因子-BB（platelet-derived growth factor-BB，PDGF-BB）是 DOR 组（7.34±16.11 pg/mL）明显低于 NOR 组（24.39±41.38 pg/mL）（p=0.005）的唯一细胞因子，与实际年龄无关。