School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, 730070, PR China.
School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, 730070, PR China.
Ecotoxicol Environ Saf. 2020 Apr 1;192:110250. doi: 10.1016/j.ecoenv.2020.110250. Epub 2020 Feb 3.
A bacterial strain designated Lysinibacillus fusiformis 15-4 was isolated from oil-free soil on the Qinghai-Tibet Plateau, which can grow well utilizing petroleum hydrocarbons as a carbon source at a lower temperature. To deeply characterize the molecular adaptations and metabolic processes of this strain when grown in a petroleum-containing environment, transcriptome analysis was performed. A total of 4664 genes and the expression of 3969 genes were observed in strain 15-4. When the strain was grown in petroleum-containing medium, 2192 genes were significantly regulated, of which 1312 (60%) were upregulated and 880 (40%) were downregulated. This strain degraded and adapted to petroleum via modulation of diverse molecular processes, including improvements in transporter activity, oxidoreductase/dehydrogenase activity, two-component system/signal transduction, transcriptional regulation, fatty acid catabolism, amino acid metabolism, and environmental stress responses. Many strain-specific genes were involved in the oxidation of hydrocarbon compounds, such as several luciferase family alkane monooxygenase genes, flavin-utilizing monooxygenase family genes, and flavoprotein-like family alkanesulfonate monooxygenase genes. Several cold shock protein genes were also induced suggesting adaptation to cold environments and the potential for petroleum degradation at low temperatures. The results obtained in this study may broaden our understanding of molecular adaptation of bacteria to hydrocarbon-containing environments and may provide valuable data for further study of L. fusiformis.
一株名为溶杆菌 15-4 的细菌菌株从青藏高原无油土壤中分离得到，它可以在较低温度下很好地利用石油烃作为碳源生长。为了深入研究该菌株在含油环境中的分子适应和代谢过程，进行了转录组分析。在 15-4 菌株中观察到 4664 个基因和 3969 个基因的表达。当菌株在含油培养基中生长时，有 2192 个基因被显著调控，其中 1312 个（60%）上调，880 个（40%）下调。该菌株通过调节多种分子过程来降解和适应石油，包括提高转运蛋白活性、氧化还原酶/脱氢酶活性、双组分系统/信号转导、转录调控、脂肪酸分解代谢、氨基酸代谢和环境应激反应。许多菌株特异性基因参与烃类化合物的氧化，如几个荧光素家族烷烃单加氧酶基因、黄素利用单加氧酶家族基因和黄素蛋白样家族烷烃磺酸盐单加氧酶基因。还诱导了几个冷休克蛋白基因，表明该菌株适应寒冷环境，并且可能在低温下进行石油降解。本研究的结果可能会拓宽我们对细菌适应含烃环境的分子适应的理解，并为进一步研究溶杆菌提供有价值的数据。
