Marmitt Marcela, Cauduro Guilherme Pinto, Sbruzzi Renan César, Valiati Victor Hugo
Laboratory of Genetics and Molecular Biology, University of Vale Do Rio Dos Sinos, São Leopoldo, Rio Grande Do Sul, Brazil.
Laboratory of Immunogenetics, Federal University of Rio Grande Do Sul, Porto Alegre, Rio Grande Do Sul, Brazil.
Mol Biotechnol. 2024 Sep 19. doi: 10.1007/s12033-024-01284-6.
Bacteria-mediated bioremediation is widely employed for its environmental benefits. The genus Burkholderia can degrade persistent organic compounds, however, little is known about its mechanisms. To increase this knowledge, Burkholderia vietnamiensis G4 bacteria were exposed to benzo[a]pyrene, a recalcitrant compound, and the expression of twelve genes of interest was analyzed at 1, 12 and 24 h. In addition, benzo[a]pyrene degradation, evaluation of cell viability and fluorescence emission of assimilated benzo[a]pyrene was performed over 28 days. The up-regulated genes were xre, paaE, livG and pckA at the three times, ACAD, atoB, bmoA and proV at 1 h and AstB at 12 h. These genes are important for bacterial survival in stress situations, breakdown and metabolization of organic compounds, and nutrient transport and uptake. Furthermore, a 52% reduction of the pollutant was observed, there was no significant variation in the viability rate of the cells, and fluorescence indicated an accumulation of benzo[a]pyrene after 24 h. Our study demonstrates the bacteria adaptability and ability to modulate the expression of genes at different times and as needed. This increases our understanding of biodegradation processes and opens new possibilities for using this bacterial strain as a tool for the bioremediation of contaminated areas.
细菌介导的生物修复因其环境效益而被广泛应用。伯克霍尔德氏菌属能够降解持久性有机化合物，然而，其降解机制却鲜为人知。为了增进对这方面的了解，将越南伯克霍尔德氏菌G4暴露于难降解化合物苯并[a]芘中，并在1小时、12小时和24小时分析了12个目标基因的表达情况。此外，在28天内对苯并[a]芘的降解、细胞活力评估以及同化苯并[a]芘的荧光发射进行了检测。在这三个时间点上调的基因有xre、paaE、livG和pckA，在1小时上调的有ACAD、atoB、bmoA和proV，在12小时上调的是AstB。这些基因对于细菌在应激情况下的存活、有机化合物的分解和代谢以及营养物质的运输和摄取都很重要。此外，观察到污染物减少了52%，细胞活力率没有显著变化，并且荧光显示24小时后苯并[a]芘有所积累。我们的研究证明了细菌的适应性以及根据需要在不同时间调节基因表达的能力。这增进了我们对生物降解过程的理解，并为将这种细菌菌株用作污染区域生物修复工具开辟了新的可能性。
