Civil Engineering, College of Science and Engineering, National University of Ireland Galway, Ireland; Ryan Institute, National University of Ireland Galway, Ireland; MaREI, The SFI Research Centre for Energy, Climate and Marine, Ireland.
Civil Engineering, College of Science and Engineering, National University of Ireland Galway, Ireland; Ryan Institute, National University of Ireland Galway, Ireland.
Waste Manag. 2021 May 1;126:152-162. doi: 10.1016/j.wasman.2021.03.011. Epub 2021 Mar 23.
This study investigated the performance of anaerobic co-digestion (AcoD) of pig manure and food waste on the reduction of antibiotic resistomes under wet and dry AcoD conditions. High-throughput quantitative PCR technology was utilized for a comprehensive assessment of the performances of the two processes. The results show that dry AcoD with a total solids (TS) content of 20% effectively reduced total antibiotic resistance genes (ARGs) by 1.24 log copies/g wet sample, while only 0.54 log copies/g wet sample was reduced in wet AcoD with a TS content of 5%. Dry AcoD was more efficient in reduction of aminoglycosides, multidrug and sulfonamide resistance genes compared with the reduction of other classes of ARGs. Dry AcoD caused a significant reduction of ARGs with resistance mechanisms of efflux pump and antibiotic deactivation. In contrast, there was no obvious difference in reductions of ARGs with different resistance mechanisms in wet AcoD. Network analysis showed that ARGs were significantly correlated with mobile genetic elements (MGEs) (Spearman's r > 0.8, P < 0.05), as well as microbial communities. Enrichment of ARGs and MGEs was found at the early period of AcoD processes, indicating some ARGs and MGEs increased during the hydrolysis and acidogenesis stages. But after a long retention time, their abundances were effectively reduced by dry AcoD in the subsequent stages.
本研究考察了猪粪和食物垃圾在湿式和干式厌氧共消化（AcoD）条件下减少抗生素抗性组的性能。高通量定量 PCR 技术用于全面评估这两种工艺的性能。结果表明，总固体（TS）含量为 20%的干式 AcoD 可有效减少 1.24 对数拷贝/克湿样品的总抗生素抗性基因（ARGs），而 TS 含量为 5%的湿式 AcoD 仅减少 0.54 对数拷贝/克湿样品。与其他 ARG 类别的减少相比，干式 AcoD 更有效地减少氨基糖苷类、多药和磺胺类抗性基因。干式 AcoD 导致具有外排泵和抗生素失活抗性机制的 ARGs 显著减少。相比之下，湿式 AcoD 中不同抗性机制的 ARGs 减少没有明显差异。网络分析表明，ARGs 与可移动遗传元件（MGEs）（Spearman's r>0.8，P<0.05）以及微生物群落显著相关。在 AcoD 过程的早期发现 ARGs 和 MGEs 富集，表明在水解和产酸阶段，一些 ARGs 和 MGEs 增加。但经过长时间的保留时间后，它们的丰度在随后的阶段被干式 AcoD 有效降低。
