Robinson Sean, Kramer Jonathan, Shelton Trevor, Merriman Jarrad, Haus Brian
San Francisco Orthopedic Residency Program, San Francisco.
UC Davis Medical Center.
J Pediatr Orthop. 2020 Mar;40(3):110-113. doi: 10.1097/BPO.0000000000001181.
The goal of this study is to determine whether harvested cartilage from an osteochondral loose body maintains the same viability for implantation as cartilage harvested from the traditional locations within the adolescent knee for autologous chondrocyte implantation (ACI).
A retrospective study was performed on all ACI procedures performed from 2014 to 2017 at a single institution. Biopsies were derived from 2 groups: osteochondral loose body verses the intercondylar notch. The viability, yield, identity, potency, and density were obtained from each sample in addition to basic demographics and concomitant injuries. A total of 12 patients with osteochondral loose bodies 14.6 (SD=2.9) and 20 patients 13.6 (SD=3.3) with intercondylar notch biopsies were evaluated for the study.
In the microscopic and histologic comparison, there was no significant difference in viability: 94% in the loose bodies and 93% in the intercondylar notch groups, identity: 7.4 d5L versus 6.3 d5L, or yield. Minimum yield is presented as different units in Carticel (1.2×10 cells/vial) and matrix-induced ACI (>8500 relative fluorescent units) products; however, there was no difference between groups and all samples were above the acceptable limit. Minimum identity value is recorded as d5L> -2.00 and all samples were above this limit. In addition, no sample had signs of contamination or endotoxin in either group.
These results demonstrate an alternative method for obtaining cartilage biopsies in ACI procedures that may limit short-term and long-term donor site morbidity.
Level III.
本研究的目的是确定从骨软骨游离体获取的软骨在用于自体软骨细胞移植（ACI）时，其植入后的存活能力是否与从青少年膝关节传统部位获取的软骨相同。
对2014年至2017年在单一机构进行的所有ACI手术进行回顾性研究。活检样本来自两组：骨软骨游离体组和髁间切迹组。除了基本人口统计学数据和伴随损伤外，还从每个样本中获取了存活能力、产量、特性、效能和密度数据。共有12例骨软骨游离体患者（平均年龄14.6岁，标准差2.9岁）和20例髁间切迹活检患者（平均年龄13.6岁，标准差3.3岁）纳入本研究。
在显微镜和组织学比较中，存活能力无显著差异：游离体组为94%，髁间切迹组为93%；特性方面：游离体组为7.4 d5L，髁间切迹组为6.3 d5L；产量方面也无差异。在Carticel产品（1.2×10细胞/瓶）和基质诱导ACI产品（>8500相对荧光单位）中，最低产量以不同单位表示；然而，两组之间无差异，所有样本均高于可接受限度。最低特性值记录为d5L > -2.00，所有样本均高于此限度。此外，两组中均无样本有污染或内毒素迹象。
这些结果证明了在ACI手术中获取软骨活检的一种替代方法，该方法可能会减少短期和长期供区并发症。
三级。
