Unit of Research for Practical Application, Korea Polar Research Institute, Incheon 21990, Korea.
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
Genes (Basel). 2020 Feb 3;11(2):160. doi: 10.3390/genes11020160.
The southern elephant seal is the largest phocid seal and one of the two species of elephant seals. They are listed as 'least concern' by the International Union for Conservation of Nature (IUCN) Red List of Threatened Species 2015. Here, we have assembled the reference genome for using the 10× chromium sequencing platform. The final genome assembly of was 2.42 Gb long, with a contig N50 length of 54 Mb and a maximum length of 111.6 Mb. The genome contained 20,457 predicted protein-coding genes and possessed 41.51% repeated sequences. The completeness of the genome was evaluated using benchmarking universal single-copy orthologous genes (BUSCOs): the assembly was highly complete, containing 95.6% of the core set of mammalian genes. The high-quality genomic information on will be essential for further understanding of adaptive metabolism upon repeated breath-hold dives and the exploration of molecular mechanisms contributing to its unique biochemical and physiological characteristics. The southern elephant seal genome project was deposited at NCBI (National Center for Biotechnology Information) under BioProject number PRJNA587380.
南方象海豹是最大的海豹之一,也是象海豹的两个物种之一。它们被国际自然保护联盟(IUCN)濒危物种红色名录 2015 列为“关注最少”。在这里,我们使用 10×chromium 测序平台为 组装了参考基因组。最终的 基因组组装长度为 24.2 亿 bp,N50 长度为 54Mb,最大长度为 111.6Mb。 基因组包含 20457 个预测的蛋白质编码基因,并且具有 41.51%的重复序列。使用基准通用单拷贝直系同源基因(BUSCOs)评估了 的基因组完整性:该组装非常完整,包含了核心哺乳动物基因的 95.6%。高质量的 基因组信息对于进一步了解其在反复潜水呼吸暂停时的适应性代谢以及探索导致其独特生化和生理特征的分子机制至关重要。南方象海豹基因组计划已在 NCBI(美国国家生物技术信息中心)下的 BioProject 编号 PRJNA587380 中进行了存储。