College of Pharmacy, Zhejiang University of Technology, Hangzhou, China.
J Biomater Sci Polym Ed. 2020 Apr;31(6):804-815. doi: 10.1080/09205063.2020.1719684. Epub 2020 Feb 7.
The aim of this study was to develop a new chitosan derivative and investigate its effects on fresh tissue healing in rats. A chitosan-fructose Schiff based quaternary ammonium salt (CS = Fru-DEAE) was synthesized for the first time and characterized using FT-IR and HNMR, and the modification rate and the solution properties were studied. A rat wound model was established, and the experimental group was treated using 0.1 g of the chitosan derivative hydrogel. The wound healing rate, and the concentration of collagen III and proline in the wounds were assessed in the experimental group and compared with those of the control group at 7, 10, and 15 d. The CS = Fru-DEAE hydrogel demonstrated good performance and promoted the healing of infected wounds in rats. The hydrogel could accelerate the infiltration of inflammatory cells and increase the amount of type III collagen in the wound area, which likely contributed to its efficacy.
本研究旨在开发一种新型壳聚糖衍生物，并研究其对大鼠新鲜组织愈合的影响。首次合成了壳聚糖-果糖席夫碱季铵盐（CS = Fru-DEAE），并通过傅里叶变换红外光谱（FT-IR）和核磁共振氢谱（HNMR）进行了表征，同时研究了其修饰率和溶液性质。建立了大鼠创面模型，实验组采用 0.1g 壳聚糖衍生物水凝胶进行治疗。在第 7、10 和 15 天，评估实验组和对照组的创面愈合率以及创面中 III 型胶原和脯氨酸的浓度。CS = Fru-DEAE 水凝胶表现出良好的性能，可促进大鼠感染创面的愈合。水凝胶可加速炎症细胞的浸润，并增加创面区域 III 型胶原的含量，这可能是其疗效的原因。
