Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, Sfax, Tunisia.
Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, Sfax, Tunisia.
Mater Sci Eng C Mater Biol Appl. 2020 Aug;113:110978. doi: 10.1016/j.msec.2020.110978. Epub 2020 Apr 18.
This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing. By increasing the concentration of the protein isolate, chitosan hydrogels were more elastic at a protein isolate concentration not exceeding 15% (w/w). Chitosan-protein isolate composite hydrogels revealed low cytotoxicity towards MG-63 osteosarcoma cells. Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study. Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0). Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities. The in vivo healing potential of hydrogels in rats' models was further studied. Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
本工作旨在开发壳聚糖和蛋白质分离物复合水凝胶，用于控制类胡萝卜素的递送和伤口愈合。通过增加蛋白质分离物的浓度，壳聚糖水凝胶在蛋白质分离物浓度不超过 15%（w/w）时更具弹性。壳聚糖-蛋白质分离物复合水凝胶对 MG-63 骨肉瘤细胞表现出低细胞毒性。由于其适当的结构、溶胀和机械阻力特性，壳聚糖水凝胶（3%；w/v），用 15%（w/w）的蛋白质分离物增强，被选择用于类胡萝卜素的体外释放研究。释放曲线显示了递送模式，与更酸性的微环境（pH 4.0 和 pH 2.0）相比，类胡萝卜素在 pH 7.4 介质（p<.05）中更难以释放。因此，开发的水凝胶可用作 pH 敏感的智能载体，用于药物控制释放，并具有有趣的抗氧化能力。水凝胶在大鼠模型中的体内愈合潜力进一步得到了研究。水凝胶基贴片的局部应用允许加速伤口愈合，并使富含类胡萝卜素的复合水凝胶完全愈合。
