微生物底盘的天然产物生物合成开发。
Microbial Chassis Development for Natural Product Biosynthesis.
机构信息
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
出版信息
Trends Biotechnol. 2020 Jul;38(7):779-796. doi: 10.1016/j.tibtech.2020.01.002. Epub 2020 Feb 3.
Engineering microbial cells to efficiently synthesize high-value-added natural products has received increasing attention in recent years. In this review, we describe the pipeline to build chassis cells for natural product production. First, we discuss recently developed genome mining strategies for identifying and designing biosynthetic modules and compare the characteristics of different host microbes. Then, we summarize state-of-the-art systems metabolic engineering tools for reconstructing and fine-tuning biosynthetic pathways and transport mechanisms. Finally, we discuss the future prospects of building next-generation chassis cells for the production of natural products. This review provides theoretical guidance for the rational design and construction of microbial strains to produce natural products.
近年来，工程化微生物细胞以高效合成高附加值天然产物受到了越来越多的关注。在这篇综述中，我们描述了用于构建天然产物生产底盘细胞的流水线。首先，我们讨论了最近开发的基因组挖掘策略，用于鉴定和设计生物合成模块，并比较了不同宿主微生物的特点。然后，我们总结了用于重建和微调生物合成途径和运输机制的最先进的系统代谢工程工具。最后，我们讨论了构建用于生产天然产物的新一代底盘细胞的未来前景。本文综述为合理设计和构建生产天然产物的微生物菌株提供了理论指导。
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