The East Coast Research Institute of Life Science, Gangneung-Wonju National University, Gangneung, 25457, South Korea.
Department of Marine Biotechnology, Gangneung-Wonju National University, Gangneung, 25457, South Korea.
Sci Rep. 2020 Feb 6;10(1):1987. doi: 10.1038/s41598-020-58915-6.
Salmonid fishes, chum salmon (Oncorhynchus keta) have the developed adaptive strategy to withstand wide salinity changes from the early life stage. This study investigated gene expression patterns of cell membrane proteins in the gill of chum salmon fry on the transcriptome level by tracking the salinity acclimation of the fish in changing environments ranging from freshwater (0 ppt) to brackish water (17.5 ppt) to seawater (35 ppt). Using GO analysis of DEGs, the known osmoregulatory genes and their functional groups such as ion transport, transmembrane transporter activity and metal ion binding were identified. The expression patterns of membrane protein genes, including pump-mediated protein (NKA, CFTR), carrier-mediated protein (NKCC, NHE3) and channel-mediated protein (AQP) were similar to those of other salmonid fishes in the smolt or adult stages. Based on the protein-protein interaction analysis between transmembrane proteins and other related genes, we identified osmotic-related genes expressed with salinity changes and analyzed their expression patterns. The findings of this study may facilitate the disentangling of the genetic basis of chum salmon and better able an understanding of the osmophysiology of the species.
鲑鱼，包括大麻哈鱼（Oncorhynchus keta），具有发达的适应性策略，可以承受从早期生命阶段开始的广泛盐度变化。本研究通过跟踪大麻哈鱼鱼苗在从淡水（0 ppt）到半咸水（17.5 ppt）到海水（35 ppt）的变化环境中的盐度适应情况，在转录组水平上研究了鲑鱼鱼苗鳃中细胞膜蛋白的基因表达模式。通过对 DEGs 的 GO 分析，鉴定了已知的渗透压调节基因及其功能群，如离子转运、跨膜转运体活性和金属离子结合。膜蛋白基因的表达模式，包括泵介导的蛋白（NKA、CFTR）、载体介导的蛋白（NKCC、NHE3）和通道介导的蛋白（AQP），与其他鲑鱼在幼鱼或成鱼阶段的表达模式相似。基于跨膜蛋白与其他相关基因之间的蛋白质-蛋白质相互作用分析，我们确定了与盐度变化相关的表达基因，并分析了它们的表达模式。本研究的结果可能有助于阐明大麻哈鱼的遗传基础，并更好地了解该物种的渗透生理学。
