Efficiency of the () fungal formulation to control in vivo and in vitro of and in sheep.

The objective of the present work was to evaluate the efficiency of Bioverm fungal formulation (-AC001) in controlling and in sheep. In vitro predation tests were carried out in Petri dishes containing agar culture medium 2%. Four experimental groups were formed, with five replicates each: Group 1: 1 g of and 1000 third-stage larvae (L) of ; Group 2: 1000 L of ; Group 3: 1 g of and 1000 L of ; and Group 4: 1000 L of . In the in vivo tests, twelve 11-month-old sheep males positive for were used. The animals were sorted in two groups (treatment and control), based on fecal egg counts (eggs per gram, EPG). Each group comprised six animals: treatment group-each animal received orally 100 g of ; and control group-each animal received orally 100 g of rice. Subsequently, feces from these animals were collected at 12, 24, 36, 48, 60, 72, 84, and 96 h after administration. In vitro results demonstrate that kept its predatory activity with 91.5% of mean reduction percentage of L. After the passage test, presented efficacy already after 12 h of its administration and kept similar results for 60 h. Bioverm fungal formulation (-AC001) was efficient in reducing the population of and under laboratory conditions in sheep feces. However, further studies are needed under natural conditions of ruminant grazing to prove the efficiency of this product.