Ma Yuan, Jiang Lili, Fan Zhaobin, Hao Luyao, Li Zhengyi, Zhang Yanni, Li Qiannan, Wang Rui, Luo Hongliang
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, People's Republic of China.
Key Laboratory of Clinical Diagnosis and Treatment of Animal Diseases, Ministry of Agriculture, National Animal Medicine Experimental Teaching Center, Beijing, People's Republic of China.
Sci Rep. 2025 Jan 13;15(1):1843. doi: 10.1038/s41598-025-85844-z.
Duddingtonia flagrans is a nematode-trapping fungus that is widely used to control parasitic nematodes in livestock. After oral ingestion and passage through the digestive tract of animals, this microorganism captures nematodes in feces. Although many researchers have examined the safety of this fungus for humans, animals, and the environment, few reports have discussed the safety of nematode-trapping D. flagrans biologics for animals. In this study, D. flagrans safety was tested, while adverse effects and toxicities were examined in sheep. First, the nematode killing effects in naturally parasitized sheep after administration of lyophilized D. flagrans preparations were tested.lyophilized D. flagrans preparations were administered to sheep at various doses, followed by key blood factor monitoring and an examination of major tissues, organ lesions, and pathology. Lastly, lyophilized D. flagrans preparations were administered to sheep at various doses, followed by key blood factor monitoring and an examination of major tissues, organ lesions, and pathology. the nematode killing effects of naturally parasitized sheep after administration were tested. The results demonstrated that treatment with D. flagrans isolates significantly reduced developing larvae numbers in feces, with an efficiency of 92.99%. Lyophilized preparations had no observable effects on physiological parameters in sheep, thus indicating a wide safety range in target animals, with potentially minimal risks in veterinary clinical practice. Overall, D. flagrans freeze-dried biologics effectively helped to controlled parasitic infections, which are safe in animals like sheep, and thus may provide a practical platform for nematode-trapping fungi in veterinary clinical settings.
弗氏节丛孢是一种捕食线虫真菌，广泛用于控制家畜体内的寄生线虫。经动物口服并通过消化道后，这种微生物会在粪便中捕获线虫。尽管许多研究人员已研究了这种真菌对人类、动物和环境的安全性，但很少有报告讨论捕食线虫的弗氏节丛孢生物制品对动物的安全性。在本研究中，对弗氏节丛孢的安全性进行了测试，并在绵羊中检查了其不良反应和毒性。首先，测试了冻干的弗氏节丛孢制剂给药后对自然感染寄生虫的绵羊的杀线虫效果。将冻干的弗氏节丛孢制剂以不同剂量给绵羊给药，随后监测关键血液因子并检查主要组织、器官病变和病理学情况。最后，将冻干的弗氏节丛孢制剂以不同剂量给绵羊给药，随后监测关键血液因子并检查主要组织、器官病变和病理学情况。测试了给药后自然感染寄生虫的绵羊的杀线虫效果。结果表明，用弗氏节丛孢分离株进行处理可显著减少粪便中发育幼虫的数量，效率为92.99%。冻干制剂对绵羊的生理参数没有明显影响，因此表明在目标动物中的安全范围很广，在兽医临床实践中的潜在风险可能最小。总体而言，弗氏节丛孢冻干生物制品有效地帮助控制了寄生虫感染在绵羊等动物中是安全的因此可能为兽医临床环境中的捕食线虫真菌提供一个实用平台。
