Shangguan Lingfei, Chen Mengxia, Fang Xiang, Xie Zhenqiang, Zhang Kekun, Zheng Ting, Pu Yunfeng, Fang Jinggui
1Department of Horticulture, Nanjing Agricultural University, Nanjing, 210095 Jiangsu People's Republic of China.
Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, 210095 People's Republic of China.
3 Biotech. 2020 Feb;10(2):72. doi: 10.1007/s13205-019-2039-3. Epub 2020 Jan 27.
Bud dormancy is one of the most important defensive mechanisms through which plants resist cold stress during harsh winter weather. , , and have been reported to be involved in many biological processes, including bud dormancy. In the present study, grapevine () and other thirteen plants (six woody plants and seven herbaceous plants) were analyzed for the quantity, sequence structure, and evolution patterns of their , , and gene family members. Moreover, the expression of , , and genes was also investigated. Thus, 51 , 1,205 , and 489 genes were isolated from selected genomes, while 5 , 114 , and 50 duplicate gene pairs were identified in 10 genomes. Moreover, WGD and segmental duplication events were associated with the majority of the expansions of and gene families. The , , and genes significantly differentially expressed throughout bud dormancy outnumbered those significantly differentially expressed throughout fruit development or under abiotic stresses. Interestingly, multiple stress responsive genes were identified, such as (VIT_00s0313g00070), two genes (VIT_18s0001g11310 and VIT_02s0025g02250), and two genes (VIT_07s0031g01710 and VIT_11s0052g00450). These data provide candidate genes for molecular biology research investigating bud dormancy and responses to abiotic stresses (namely salt, drought, copper, and waterlogging).
芽休眠是植物在严寒冬季抵御低温胁迫的最重要防御机制之一。据报道，[具体基因名称未给出]参与了包括芽休眠在内的许多生物学过程。在本研究中，对葡萄（[葡萄品种未给出]）和其他13种植物（6种木本植物和7种草本植物）的[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]基因家族成员的数量、序列结构和进化模式进行了分析。此外，还研究了[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]基因的表达情况。因此，从选定的基因组中分离出了51个[具体基因名称未给出]、1205个[具体基因名称未给出]和489个[具体基因名称未给出]基因，同时在10个基因组中鉴定出了5个[具体基因名称未给出]、114个[具体基因名称未给出]和50个[具体基因名称未给出]重复基因对。此外，全基因组复制（WGD）和片段重复事件与[具体基因名称未给出]和[具体基因名称未给出]基因家族的大多数扩增有关。在整个芽休眠过程中显著差异表达的[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]基因数量超过了在整个果实发育过程中或非生物胁迫下显著差异表达的基因数量。有趣的是，鉴定出了多个胁迫响应基因，如[具体基因名称未给出]（VIT_00s0313g00070）、两个[具体基因名称未给出]基因（VIT_18s0001g11310和VIT_02s0025g02250）以及两个[具体基因名称未给出]基因（VIT_07s0031g01710和VIT_11s0052g00450）。这些数据为研究芽休眠和非生物胁迫（即盐、干旱、铜和涝害）响应的分子生物学研究提供了候选基因。
