Department of Physiology, Institute of Nutrition and Food Technology, Centre for Biomedical Research, Health and Sport Research Centre (IMUDS), Universidad de Granada, Granada, Spain.
Department of Pharmacology, School of Pharmacy, Biohealth Research Institute, Centre for Biomedical Research, Universidad de Granada, Campus Universitario de Cartuja s/n, 18071, Granada, Spain.
Eur J Nutr. 2020 Dec;59(8):3473-3490. doi: 10.1007/s00394-020-02181-9. Epub 2020 Feb 6.
Functional and structural changes in cardiovascular and renal systems resulting from obesity and metabolic syndrome represent a severe risk to human health. Lifestyle interventions such as combining healthy diet with adequate physical exercise protocols are good strategies to manage these pathologies. In this research, the effects of lentil protein hydrolysate administration, combined or not with a mixed training protocol, on insulin resistance, cardiovascular and renal functionality were studied in the obese Zucker rat experimental model.
Thirty-two rats (16 lean and 16 obese subdivided in sedentary and trained animals) were administered lentil protein hydrolysate, whereas another 32 subdivided in the same experimental design were administered placebo. The experiment lasted for 8 weeks. At the end of the experimental period, insulin resistance and different parameters of cardiovascular and renal functionality were measured.
The individual or combined interventions with lentil protein hydrolysate and mixed training protocol were efficient at counteracting some of the metabolic, cardiovascular and renal alterations characterizing the obese Zucker rat. Specifically, lentil protein hydrolysate decreased hyperphagia, amplitude of QRS complex, plasma ACE and selectin E expression in aorta, while increasing urinary volume and pH. Exercise showed beneficial actions on HOMA-IR, QRS amplitude, QTc interval, urinary volume, kidney weight and Mn-SOD activity. Interestingly, most of the mentioned benefits of exercise were more consistent when protein hydrolysate was also administered.
The interesting synergies between the two interventions assessed qualify them as alternative therapeutic strategies to treat cardiovascular and kidney diseases associated to the metabolic syndrome.
肥胖和代谢综合征引起的心血管和肾脏系统的功能和结构变化对人类健康构成严重威胁。将健康饮食与充足的体育锻炼方案相结合的生活方式干预是管理这些病理的良好策略。在这项研究中，研究了食用扁豆蛋白水解物，结合或不结合混合训练方案，对肥胖 Zucker 大鼠实验模型中胰岛素抵抗、心血管和肾功能的影响。
32 只大鼠（16 只瘦鼠和 16 只肥胖鼠，再细分为久坐不动和训练的动物）给予扁豆蛋白水解物，而另外 32 只大鼠按相同的实验设计分为给予安慰剂。实验持续 8 周。在实验期末，测量胰岛素抵抗和心血管及肾功能的不同参数。
单独或联合使用扁豆蛋白水解物和混合训练方案的干预措施有效地对抗了肥胖 Zucker 大鼠的一些代谢、心血管和肾脏改变。具体来说，扁豆蛋白水解物减少了多食、QRS 复合波振幅、血浆 ACE 和主动脉选择素 E 的表达，同时增加了尿量和 pH 值。运动对 HOMA-IR、QRS 振幅、QTc 间期、尿量、肾脏重量和 Mn-SOD 活性有有益作用。有趣的是，当同时给予蛋白水解物时，运动的大部分益处更为一致。
评估的两种干预措施之间的有趣协同作用使它们成为治疗与代谢综合征相关的心血管和肾脏疾病的替代治疗策略。
