Instituto de Investigación en Ciencias de Alimentación (CIAL, CSIC-UAM), Madrid 28049, Spain.
Grupo de Investigación en Nutrición y Farmacología (URJC), Unidad Asociada al Instituto de Investigación en Ciencias de la Alimentación (CSIC), Madrid 28049, Spain.
Nutrients. 2018 Apr 3;10(4):441. doi: 10.3390/nu10040441.
The purpose of this study was to evaluate the effect of the administration of two egg white hydrolysates on glucose metabolism complications related to Metabolic Syndrome (MS) in Zucker fatty rats (ZFR). ZFR were given 750 mg/kg/day of egg white hydrolyzed with pepsin (HEW1) or with aminopeptidase (HEW2) for 12 weeks in their drinking water or just water. Zucker lean rats (ZLR), which received water, were used as a control. The presence of tactile allodynia, which is a sign of peripheral neuropathy, was assessed. Blood samples and pancreas were collected to determine the effect of the hydrolysates on glucose metabolism. The intake of HEW1 significantly lowered plasma insulin levels and improved the quantitative indexes of insulin resistance, insulin sensitivity, and pancreatic β-cell functionality (HOMA-IR, HOMA-β, and QUICKI, respectively), but non-significant changes were observed in group treated with HEW2. Compared to ZLR, ZFR showed tactile allodynia, but the consumption of both hydrolysates significantly increased mechanical sensitivity in ZFR. In conclusion, HEW1 pepsin could improve the glucose metabolism abnormalities associated with MS in obese Zucker rats.
本研究旨在评估两种蛋清水解物对代谢综合征（MS）相关葡萄糖代谢并发症的影响。ZFR 连续 12 周通过饮用水给予 750mg/kg/天的胃蛋白酶水解蛋清（HEW1）或氨肽酶水解蛋清（HEW2），或仅给予水。将接受水的 Zucker 瘦鼠（ZLR）作为对照。评估是否存在触觉过敏，这是周围神经病变的一个迹象。收集血液样本和胰腺，以确定水解物对葡萄糖代谢的影响。HEW1 的摄入显著降低了血浆胰岛素水平，并改善了胰岛素抵抗、胰岛素敏感性和胰岛β细胞功能的定量指标（HOMA-IR、HOMA-β 和 QUICKI），但用 HEW2 治疗的组未观察到显著变化。与 ZLR 相比，ZFR 表现出触觉过敏，但两种水解物的消耗都显著增加了 ZFR 的机械敏感性。总之，胃蛋白酶 HEW1 可以改善肥胖 Zucker 大鼠与 MS 相关的葡萄糖代谢异常。
