Konev V V, Popov G A
Biokhimiia. 1988 Sep;53(9):1439-42.
In the presence of intact Ehrlich ascite carcinoma cells and the supernatant obtained by preincubation and subsequent precipitation of cells, egg phosphatidylcholine is oxidized in liposomes to form malonic dialdehyde (MDA). Catalase and carbon dioxide markedly reduce, whereas sodium azide increases MDA accumulation during liposome incubation with the cells. EDTA, diethylthiocarbonate and alpha-tocopherol effectively inhibit, whereas ascorbate and cysteine strongly activate MDA synthesis in both cases. Superoxide dismutase has no appreciable effect on these processes. It is concluded that metal-containing catalysts and the H2O2 released by intact cells into the incubation medium induce lipid peroxidation in liposomes.
在存在完整的艾氏腹水癌细胞以及通过细胞预孵育和随后沉淀获得的上清液的情况下，卵磷脂在脂质体中被氧化形成丙二醛（MDA）。过氧化氢酶和二氧化碳可显著减少MDA的生成，而叠氮化钠在脂质体与细胞孵育期间会增加MDA的积累。在这两种情况下，乙二胺四乙酸（EDTA）、二乙基硫代碳酸盐和α-生育酚可有效抑制MDA合成，而抗坏血酸和半胱氨酸则强烈激活MDA合成。超氧化物歧化酶对这些过程没有明显影响。得出的结论是，含金属催化剂以及完整细胞释放到孵育培养基中的过氧化氢会诱导脂质体中的脂质过氧化。
