Babizhaev M A, Aĭtmagambetov M T, Deev A I, Vladimirov Iu A
Biull Eksp Biol Med. 1987 Jan;103(1):38-40.
The level of lipid peroxidation products (LPP) was determined in the aqueous humor from the anterior chamber of patients with cataract and donor eyes. The content of LPP in senile cataract aqueous humor was shown to be significantly increased. To determine the possible mechanism of LPP increase in aqueous humor, human lenses at different stages of cataract as well as transparent human and rabbit lenses were incubated for 3 hours in 3.0 ml medium containing liposomes (0.5 mg/ml) prepared from phospholipids from the egg yolk and 0.14 M NaCl + 0.01 M TRIS-HCl buffer, pH 7.4). Corrections were made for phospholipid autooxidation. The level of LPP accumulation in the medium was determined by MDA assay. The rate of LPP production increased significantly in transparent lenses and in early senile cataract, as compared to controls and advanced (mature) cataracts. EDTA (1 mM), superoxide dismutase (114 u/sample), catalase (900 u/sample), chelated iron (III): Fe3+-ADP addition to the incubation medium depressed the level of LPP accumulation. This suggests the participation of Fe2+, O2-., H2O2 in the mechanism of LPP production in the lens. The induction of lipid peroxidation in the lens can be significant for leukotriene and prostaglandin synthesis in the eye.
测定了白内障患者前房房水及供体眼房水中脂质过氧化产物（LPP）的水平。结果显示，老年性白内障房水中LPP的含量显著增加。为了确定房水中LPP增加的可能机制，将处于不同白内障阶段的人晶状体以及透明的人晶状体和兔晶状体在含有由蛋黄磷脂制备的脂质体（0.5mg/ml）以及0.14M NaCl + 0.01M Tris-HCl缓冲液（pH 7.4）的3.0ml培养基中孵育3小时。对磷脂的自动氧化进行了校正。通过丙二醛（MDA）测定法确定培养基中LPP的积累水平。与对照组和晚期（成熟）白内障相比，透明晶状体和早期老年性白内障中LPP的产生速率显著增加。向孵育培养基中添加乙二胺四乙酸（EDTA，1mM）、超氧化物歧化酶（114u/样品）、过氧化氢酶（900u/样品）、螯合铁（III）：Fe3 + -ADP可降低LPP的积累水平。这表明Fe2 +、O2 - 、H2O2参与了晶状体中LPP产生的机制。晶状体中脂质过氧化的诱导对于眼内白三烯和前列腺素的合成可能具有重要意义。
