Department of Energy Engineering, Future Convergence Technology Research Institute, Gyeongnam National University of Science and Technology, Jinju, Republic of Korea.
Department of Marine Bio-materials and Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National University, Busan, Republic of Korea.
Lett Appl Microbiol. 2020 Jun;70(6):431-439. doi: 10.1111/lam.13282. Epub 2020 Mar 17.
This study was performed to evaluate the effects of dietary probiotics on growth, non-specific immune responses and disease resistance in olive flounder, Paralichthys olivaceus. During 8 weeks, the fish were fed the five experimental diets such as a basal commercial diet (CON), oxytetracycline (OTC) and three basal diets containing Bacillus subtilis (BS), a commercial microbial product (CES) and a mixture of yeast and bacterium (PI), respectively. Fish fed all the probiotics diets and OTC showed a significantly higher growth than fish-fed CON (P < 0·05). Fish-fed PI had a significantly higher nitroblue tetrazolium activity, whereas fish-fed CES showed a higher lysozyme level (P < 0·05). A 7-day challenge test also showed that fish-fed PI had a cumulative survival rate equivalent to that of fish-fed OTC (P < 0·05). Moreover, the diet (PI) appeared to increase the diversity of microbial community in the fish. All these results suggest that the probiotics diet could function as a potential antibiotic replacer in the olive flounder. SIGNIFICANCE AND IMPACT OF THE STUDY: This study is unique in revealing that a diet mixture of yeast, Groenewaldozyma salmanticensis and bacterium Gluconacetobacter liquefaciens can enhance growth, innate immunity and diversity of microbial community including dominant species in the olive flounder. All these indicate that the diet mixture could function as a potential antibiotic replacer in one of the most commercially important fisheries in South Korea.
本研究旨在评估膳食益生菌对卵形鲳鲹生长、非特异性免疫反应和抗病能力的影响。在 8 周的时间里，鱼被喂食五种实验饲料，如基础商业饲料（CON）、土霉素（OTC）和三种分别含有枯草芽孢杆菌（BS）、商业微生物产品（CES）和酵母和细菌混合物（PI）的基础饲料。喂食所有益生菌饲料和 OTC 的鱼的生长速度明显高于喂食 CON 的鱼（P<0.05）。喂食 PI 的鱼的硝基四唑氮蓝活性显著较高，而喂食 CES 的鱼的溶菌酶水平较高（P<0.05）。7 天的挑战试验也表明，喂食 PI 的鱼的累积存活率与喂食 OTC 的鱼相当（P<0.05）。此外，这种饮食（PI）似乎增加了鱼体内微生物群落的多样性。所有这些结果表明，益生菌饲料可以作为卵形鲳鲹的一种潜在抗生素替代品。研究的意义和影响：本研究的独特之处在于揭示了酵母、格氏乳杆菌和细菌醋酸杆菌的饮食混合物可以提高卵形鲳鲹的生长、先天免疫和微生物群落的多样性，包括优势种。所有这些都表明，这种饮食混合物可以作为韩国最重要的渔业之一的潜在抗生素替代品。
