De Boer J M, Plantema F H
Department of Electron Microscopy and Molecular Cytology, University of Amsterdam, The Netherlands.
Can J Microbiol. 1988 Jun;34(6):757-66. doi: 10.1139/m88-129.
From patients with bacterial vaginosis motile, anaerobic, comma-shaped bacteria can be isolated, which have recently been placed into the new genus Mobiluncus. In this study, electron microscopy was used to examine the in situ adherence of these motile curved rods to detached epithelial cells (comma cells) in vaginal fluid from two patients with bacterial vaginosis. Thin sections showed that the curved rods attached both directly to the epithelial cell surface and at various distances from it. It is concluded that after initial attachment these motile bacteria can grow at the epithelial cell surface in sessile microcolonies. Ruthenium red staining demonstrated a coating of precipitated glycocalyx material both on the surface of the curved rods and on their flagella. This may indicate that in situ the adherent curved rods were enclosed in a very hydrated matrix of exopolysaccharides. Conspicuous was the ability of the curved rods to attach to the epithelial cell surface via their cell tips. However, in situ no specialized bacteria cell surface structures were seen that might explain this polar attachment. Electron microscopy of pure cultures demonstrated that both Mobiluncus curtisii subsp. curtisii and Mobiluncus mulieris can produce a glycocalyx in vitro.
从患有细菌性阴道病的患者中可分离出运动性厌氧逗号形细菌，这些细菌最近被归入新属——动弯杆菌属。在本研究中，利用电子显微镜检查了这些运动性弯曲杆菌对两名细菌性阴道病患者阴道分泌物中脱落上皮细胞（逗号细胞）的原位黏附情况。超薄切片显示，弯曲杆菌既直接附着于上皮细胞表面，也附着于距其不同距离处。得出的结论是，在初始附着后，这些运动性细菌可在上皮细胞表面以固着微菌落的形式生长。钌红染色显示，弯曲杆菌表面及其鞭毛上均有沉淀的糖萼物质涂层。这可能表明，原位的黏附弯曲杆菌被包裹在一个高度水合的胞外多糖基质中。值得注意的是，弯曲杆菌能够通过其细胞尖端附着于上皮细胞表面。然而，在原位未观察到可能解释这种极性附着的特殊细菌细胞表面结构。纯培养物的电子显微镜检查表明，柯氏动弯杆菌柯氏亚种和女性动弯杆菌在体外均可产生糖萼。
