Gerasimov Evgeny S, Zamyatnina Ksenia A, Matveeva Nadezda S, Rudenskaya Yulia A, Kraeva Natalya, Kolesnikov Alexander A, Yurchenko Vyacheslav
Faculty of Biology, M. V. Lomonosov Moscow State University, Moscow 119991, Russia.
Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov University, Moscow 119435, Russia.
Pathogens. 2020 Feb 5;9(2):100. doi: 10.3390/pathogens9020100.
Maxicircles of all kinetoplastid flagellates are functional analogs of mitochondrial genome of other eukaryotes. They consist of two distinct parts, called the coding region and the divergent region (DR). The DR is composed of highly repetitive sequences and, as such, remains the least explored segment of a trypanosomatid genome. It is extremely difficult to sequence and assemble, that is why very few full length maxicircle sequences were available until now. Using PacBio data, we assembled 17 complete maxicircles from different species of trypanosomatids. Here we present their large-scale comparative analysis and describe common patterns of DR organization in trypanosomatids.
所有动质体鞭毛虫的大环是其他真核生物线粒体基因组的功能类似物。它们由两个不同的部分组成，称为编码区和分歧区（DR）。DR由高度重复序列组成，因此仍然是锥虫基因组中研究最少的部分。对其进行测序和组装极其困难，这就是为什么到目前为止只有极少数全长大环序列可用。利用PacBio数据，我们从不同种类的锥虫中组装了17个完整的大环。在此，我们展示它们的大规模比较分析，并描述锥虫中DR组织的常见模式。
