Department of Obstetrics and Gynecology, Cruces University Hospital, Baracaldo, and Department of Medical-Surgical Specialties, University of the Basque Country Universidad del País Vasco/Euskal Herriko Universitatea, Leioa, Spain; Instituto Valenciano de Infertilidad (IVI) Bilbao, Bilbao, Spain; Biocruces, Health Research Institute, Grupo Clínico Vinculado from CIBER of Rare Diseases (CIBERER), Baracaldo, Spain.
Department of Obstetrics and Gynecology, Cruces University Hospital, Baracaldo, and Department of Medical-Surgical Specialties, University of the Basque Country Universidad del País Vasco/Euskal Herriko Universitatea, Leioa, Spain; Biocruces, Health Research Institute, Grupo Clínico Vinculado from CIBER of Rare Diseases (CIBERER), Baracaldo, Spain.
Fertil Steril. 2020 Jan;113(1):53-61. doi: 10.1016/j.fertnstert.2019.08.059.
To ascertain whether the oocytes of women who are obese or overweight have a different fatty acid (FA) profile than women with normal weight.
Prospective case-control study.
Two IVF centers.
PATIENT(S): A total of 205 women undergoing IVF and intracytoplasmic sperm injection (ICSI) were included in the study, totaling 922 oocytes.
INTERVENTION(S): The unfertilized and the immature oocytes from the women who underwent IVF/ICSI were subjected to FA analysis with capillary gas chromatography. Women were classified according their body mass index (BMI) as normal, overweight, or obese. Germinal vesicle oocytes, metaphase I oocytes, and unfertilized metaphase II oocytes were analyzed separately.
MAIN OUTCOME MEASURE(S): Fatty acid profile.
RESULT(S): A very different oocyte FA pattern was observed for each BMI. Women with normal weight had higher levels of saturated FAs, and lower levels of monosaturated FAs. Women who were obese had lower levels of n-3 polyunsaturated FA, and the lowest n-6:n-3 ratios. Regarding specific FAs, docosahexaenoic acid levels were lower in women with normal weight than in those who are overweight, and in women who are overweight than in those who are obese. The opposite occurred with eicosapentaenoic acid, with the highest levels in women who have normal weight followed by those who are overweight and lower levels in those women who were obese. When FA analysis was restricted to a subset of oocytes, many of these differences persisted.
CONCLUSION(S): Our study shows that oocytes from women who are obese or overweight have a different FA composition. This difference in levels could be related to the IVF poor outcome in these women. Therefore, this different composition could suggest that offspring of women who are obese or overweight have an unfavorable milieu even before conception.
确定肥胖或超重女性的卵子与正常体重女性的卵子的脂肪酸（FA）谱是否不同。
前瞻性病例对照研究。
两个体外受精中心。
共纳入 205 名接受体外受精和胞浆内精子注射（ICSI）的女性，共 922 个卵子。
对接受 IVF/ICSI 的女性的未受精卵和不成熟卵子进行毛细管气相色谱法 FA 分析。根据体重指数（BMI）将女性分类为正常、超重或肥胖。分别分析GV 期卵母细胞、MI 期卵母细胞和未受精的 MII 期卵母细胞。
脂肪酸谱。
每种 BMI 都观察到不同的卵母细胞 FA 模式。正常体重女性的饱和脂肪酸水平较高，单不饱和脂肪酸水平较低。肥胖女性的 n-3 多不饱和脂肪酸水平较低，n-6:n-3 比值最低。关于特定脂肪酸，正常体重女性的二十二碳六烯酸（DHA）水平低于超重女性，而超重女性的 DHA 水平又低于肥胖女性。二十碳五烯酸（EPA）则相反，正常体重女性的 EPA 水平最高，其次是超重女性，肥胖女性的 EPA 水平最低。当将 FA 分析仅限于一组卵母细胞时，许多差异仍然存在。
我们的研究表明，肥胖或超重女性的卵子具有不同的 FA 组成。这种水平上的差异可能与这些女性的 IVF 不良结局有关。因此，这种不同的组成可能表明肥胖或超重女性的后代在受孕前就处于不利的环境中。
