School of Environment and Chemical Engineering, Foshan University, Foshan, 528000, China.
School of Chemistry and Environment, South China Normal University, Guangzhou, 510631, China.
Mikrochim Acta. 2020 Feb 7;187(3):157. doi: 10.1007/s00604-020-4149-3.
The authors describe a colorimetric method for the determination of copper ions (Cu) based on anti-etching of gold nanorods (AuNRs) induced by cuprous ions (Cu). The formation of Cu from the reaction between Cu and sodium ascorbate causes the deactivation of the horseradish peroxidase (HRP), which is used as the catalyst for the reaction of 3,3',5,5'-Tetramethylbenzidine (TMB) and hydrogen peroxide (HO), resulting in a decrease in the amount of TMB product that can etch AuNRs, which is accompanied by the color changes from yellow to light orange. Thus, determination of Cu is achieved on the basis of AuNR absorbance at 450 nm. A determination limit as low as 0.28 nM at the 3S level, and the detection of 0.4 nM Cu in solution can be achieved with naked eyes, and a dynamic range of 0 to 1.6 nM toward Cu was obtained. Graphical abstractSchematic representation of a colorimetric method for determination of Cu based on the anti-etching of gold nanorods induced by Cu, which is from the reaction of Cu with sodium ascorbate.
作者描述了一种基于铜离子（Cu）诱导的金纳米棒（AuNRs）反刻蚀作用来测定铜离子（Cu）的比色法。Cu 与抗坏血酸钠反应生成的 Cu 会使辣根过氧化物酶（HRP）失活，HRP 被用作 3,3',5,5'-四甲基联苯胺（TMB）和过氧化氢（HO）反应的催化剂，导致 TMB 产物的量减少，从而无法刻蚀 AuNRs，同时伴随着颜色从黄色变为浅橙色的变化。因此，Cu 的测定是基于 AuNR 在 450nm 处的吸光度。在 3S 水平下，检测限低至 0.28nM，肉眼可检测到溶液中 0.4nM 的 Cu，对 Cu 的动态范围为 0 至 1.6nM。
