辣根过氧化物酶法检测 Cu(II)及其在比色逻辑门中的应用。
Enzymatic assay for Cu(II) with horseradish peroxidase and its application in colorimetric logic gate.
出版信息
Anal Chem. 2013 Aug 6;85(15):7029-32. doi: 10.1021/ac401925j. Epub 2013 Jul 26.
PMID:23879883
Abstract
We report an ultrasensitive and colorimetric assay for Cu(II) via enzymatic amplification strategy. The enzymatic activity of horseradish peroxidase (HRP) is strongly inhibited by Cu(I), which can be used indirectly to assay Cu(II). The limit of detection (LOD) is 0.37 nM, and the detection of 20 nM Cu(II) in solution can be achieved with naked eyes. This assay can be used to construct a colorimetric logic gate.
摘要
我们报道了一种基于酶放大策略的超灵敏比色 Cu(II) 检测方法。辣根过氧化物酶（HRP）的酶活性会被 Cu(I) 强烈抑制，因此可以间接用于检测 Cu(II)。该方法的检测限（LOD）为 0.37 nM，在溶液中对 20 nM Cu(II) 的检测可以实现肉眼观察。该方法可用于构建比色逻辑门。
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