Department of Cell Biology, Kosin University College of Medicine, 34 Amnam-dong, Seo-gu, Busan, 49267, South Korea.
Institute of Life Technology, iNtRON Biotechnology, Seongnam, South Korea.
Genes Genomics. 2020 Apr;42(4):441-447. doi: 10.1007/s13258-019-00906-7. Epub 2020 Feb 7.
Respiratory diseases in pigs are the main health concerns for swine producers. Similar to the diseases in human and other animals, respiratory diseases are primary related to morbidity and are the result of infection with bacteria, viruses, or both. B. bronchiseptica causes serious respiratory diseases in the swine airway track. However, the B. bronchiseptica-specific bacteriophage has diverse advantages such as decreasing antibiotic overuse and possible therapeutic potential against bacteria.
The objects of this study were to investigate the therapeutic effect of specific B. bronchiseptica bacteriophages and to identify genes related to bacteriophage signaling utilizing RNA microarrays in swine nasal turbinate cells.
Bor-BRP-1 phages were applied 24 h prior to B.bronchiseptica infection (1 × 10 cfu/ml) at several concentrations of bacterial infection. Cells were incubated to detect cytokines and 24 h to detect mucin production. And real-time quantitative PCR was performed to examine related genes expression. To determine the change of total gene expression based on B.bronchiseptica and Bor-BRP-1 treatment, we performed RNA sequencing experiments.
The results showed that B. bronchiseptica induced increased expression of several inflammatory genes such as IL-1β, IL-6, and Muc1 in a dose-dependent manner. However, Bor-BRP-1 induced reduction of gene expression compared to the B. bronchiseptica induction group. In addition, microarrays detected Bor-BRP-1-altered inflammatory gene expression against B. bronchiseptica, reducing B. bronchiseptica-induced airway inflammation in swine epithelial cells.
These results suggest that the specific bacteriophage has a therapeutic potential to defend against B. bronchiseptica infection by altering inflammatory gene expression profiles.
猪的呼吸系统疾病是养猪户主要关注的健康问题。与人类和其他动物的疾病类似，呼吸系统疾病主要与发病率有关，是细菌、病毒或两者共同感染的结果。支气管败血波氏杆菌会导致猪的呼吸道出现严重的疾病。然而，支气管败血波氏杆菌特异性噬菌体具有多种优势，例如减少抗生素的过度使用，以及对细菌可能具有治疗潜力。
本研究旨在利用猪鼻甲骨细胞中的 RNA 微阵列研究特定的支气管败血波氏杆菌噬菌体的治疗效果，并鉴定与噬菌体信号相关的基因。
在支气管败血波氏杆菌感染（1×10cfu/ml）前 24 小时，以几种细菌感染浓度应用 Bor-BRP-1 噬菌体。孵育细胞以检测细胞因子，并在 24 小时时检测粘蛋白的产生。并通过实时定量 PCR 检测相关基因的表达。为了确定基于支气管败血波氏杆菌和 Bor-BRP-1 处理的总基因表达变化，我们进行了 RNA 测序实验。
结果表明，支气管败血波氏杆菌以剂量依赖性方式诱导了几种炎症基因的表达增加，如 IL-1β、IL-6 和 Muc1。然而，Bor-BRP-1 的诱导作用与支气管败血波氏杆菌诱导组相比，降低了基因的表达。此外，微阵列检测到 Bor-BRP-1 改变了针对支气管败血波氏杆菌的炎症基因表达，减轻了猪上皮细胞中支气管败血波氏杆菌引起的气道炎症。
这些结果表明，特定的噬菌体通过改变炎症基因表达谱，具有抵御支气管败血波氏杆菌感染的治疗潜力。
