一种新发现的感染猪支气管败血波氏杆菌的噬菌体的特性分析。
Characterisation of a newly detected bacteriophage infecting Bordetella bronchiseptica in swine.
作者信息
Chen Yibao, Yang Lan, Sun Erchao, Song Jiaoyang, Wu Bin
机构信息
State Key laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
出版信息
Arch Virol. 2019 Jan;164(1):33-40. doi: 10.1007/s00705-018-4034-0. Epub 2018 Sep 18.
A novel virulent bacteriophage, vB_BbrM_PHB04, infecting Bordetella bronchiseptica was isolated from wastewater collected at a swine farm in China. Phage vB_BbrM_PHB04 exhibited growth over a wide range of temperature and pH conditions and showed different efficiency of plating values and lytic spectra within the same strains at 25 °C and 37 °C. High-throughput sequencing revealed that vB_BbrM_PHB04 has a linear double-stranded DNA genome with 124 putative open reading frames. Overall, the genome of vB_BbrM_PHB04 showed very low similarity (the highest nucleotide identity 82%, 1% coverage) to other phage sequences in the GenBank database. Phylogenetic analysis indicated that vB_BbrM_PHB04 is a new member of the family Myoviridae. In addition, polymerase chain reaction-based detection of phage genes in phage-resistant B. bronchiseptica variants revealed no evidence of lysogenic activity of phage vB_BbrM_PHB04.
从中国一家养猪场收集的废水中分离出一种新型烈性噬菌体vB_BbrM_PHB04，它可感染支气管败血波氏杆菌。噬菌体vB_BbrM_PHB04在广泛的温度和pH条件下均能生长，并且在25°C和37°C时，同一菌株内的平板计数效率值和裂解谱有所不同。高通量测序显示，vB_BbrM_PHB04具有线性双链DNA基因组，含有124个推定的开放阅读框。总体而言，vB_BbrM_PHB04的基因组与GenBank数据库中的其他噬菌体序列相似度极低（最高核苷酸同一性为82%，覆盖率为1%）。系统发育分析表明，vB_BbrM_PHB04是肌尾噬菌体科的一个新成员。此外，基于聚合酶链反应对噬菌体抗性支气管败血波氏杆菌变体中的噬菌体基因进行检测，结果显示没有证据表明噬菌体vB_BbrM_PHB04具有溶原活性。
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