评估滤纸条作为一种用于使用 MALDI-TOF MS 系统鉴定灭活细菌的运输手段。
Evaluation of filter paper as a means to transport inactivated bacteria for identification using the MALDI-TOF MS system.
机构信息
Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro De Pesquisa Experimental (CPE), Hospital de Clínicas de Porto Alegre (HCPA), R. Ramiro Barcelos, 2350, CEP 90035-903 Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga 2752, Porto Alegre, Brazil.
Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro De Pesquisa Experimental (CPE), Hospital de Clínicas de Porto Alegre (HCPA), R. Ramiro Barcelos, 2350, CEP 90035-903 Porto Alegre, RS, Brazil.
出版信息
J Microbiol Methods. 2020 Apr;171:105863. doi: 10.1016/j.mimet.2020.105863. Epub 2020 Feb 5.
A novel approach to transport inactivated bacteria in filter paper for identification in the MALDI-TOF MS was evaluated. Seventy four bacterial isolates were evaluated and the approach presented sensitivity of 97.3% and specificity of 100%. Inactivated bacteria in filter paper are a safer alternative to transport bacteria for MALDI-TOF MS identification.
评估了一种将滤纸上失活细菌运输到 MALDI-TOF MS 中进行鉴定的新方法。评估了 74 株细菌分离株，该方法的灵敏度为 97.3%，特异性为 100%。滤纸上的失活细菌是一种比用于 MALDI-TOF MS 鉴定的运输细菌更安全的选择。
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