Pavlovic Melanie, Wudy Corinna, Zeller-Peronnet Veronique, Maggipinto Marzena, Zimmermann Pia, Straubinger Alix, Iwobi Azuka, Märtlbauer Erwin, Busch Ulrich, Huber Ingrid
Berl Munch Tierarztl Wochenschr. 2015 Jan-Feb;128(1-2):24-30.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently emerged as a rapid and accurate identification method for bacterial species. Although it has been successfully applied for the identification of human pathogens, it has so far not been well evaluated for routine identification of veterinary bacterial isolates. This study was performed to compare and evaluate the performance of MALDI-TOF MS based identification of veterinary bacterial isolates with commercially available conventional test systems. Discrepancies of both methods were resolved by sequencing 16S rDNA and, if necessary, the infB gene for Actinobacillus isolates. A total of 375 consecutively isolated veterinary samples were collected. Among the 357 isolates (95.2%) correctly identified at the genus level by MALDI-TOF MS, 338 of them (90.1% of the total isolates) were also correctly identified at the species level. Conventional methods offered correct species identification for 319 isolates (85.1%). MALDI-TOF identification therefore offered more accurate identification of veterinary bacterial isolates. An update of the in-house mass spectra database with additional reference spectra clearly improved the identification results. In conclusion, the presented data suggest that MALDI-TOF MS is an appropriate platform for classification and identification of veterinary bacterial isolates.
基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）最近已成为一种快速准确的细菌种类鉴定方法。尽管它已成功应用于人类病原体的鉴定，但迄今为止，尚未对其在兽医细菌分离株常规鉴定中的性能进行充分评估。本研究旨在比较和评估基于MALDI-TOF MS的兽医细菌分离株鉴定与市售传统检测系统的性能。通过对16S rDNA进行测序，必要时对放线杆菌分离株的infB基因进行测序，解决了两种方法的差异。共收集了375份连续分离的兽医样本。在通过MALDI-TOF MS在属水平上正确鉴定的357株分离株（95.2%）中，其中338株（占总分离株的90.1%）在种水平上也被正确鉴定。传统方法对319株分离株（85.1%）提供了正确的种鉴定。因此，MALDI-TOF鉴定对兽医细菌分离株提供了更准确的鉴定。用额外的参考光谱更新内部质谱数据库明显改善了鉴定结果。总之，所呈现的数据表明，MALDI-TOF MS是兽医细菌分离株分类和鉴定的合适平台。
