Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia.
Department of Pharmacy, College of Medicine and Health Sciences, Adigrat University, P.O. Box 50, Adigrat, Ethiopia.
J Ethnopharmacol. 2020 May 10;253:112654. doi: 10.1016/j.jep.2020.112654. Epub 2020 Feb 5.
Capparis tomentosa Lam. root is one of the commonly used traditional medicines for the treatment of pain and inflammatory conditions by the Kunama ethnic group of Ethiopia. Yet, its ethnomedicinal claim has not been scientifically investigated.
This study aims to evaluate the anti-inflammatory and anti-nociception activities of root extracts of Capparis tomentosa Lam.
The anti-inflammatory activity of the 70% ethanolic extracts and solvent fractions was determined using invitro hyaluronidase inhibition assay and in vivo carrageenan-induced paw edema in Swiss albino mice model. The anti-nociception effect was determined using the acetic acid-induced writhing model and tail immersion model in Swiss albino mice.
The root extract of Capparis tomentosa has exhibited significant hyaluronidase inhibition activity which was comparable with the reference drug, indomethacin. Significant inhibition of carrageenan-induced paw edema was observed in all the test sample treated mice compared to the negative control with the highest activity observed from butanol fraction which was equivalent to the effect of acetylsalicylic acid. All the root extract and solvent fractions have possessed a significant and dose-dependent abdominal writhing inhibition with the highest effect observed in the aqueous fraction. Additionally, the crude extract was found better in increasing the reaction latency of the mice to the hot water stimulus than the solvent fractions which were significant after 60-120 min compared to the distilled water treated group. The root extract and solvent fractions were also found safe at a single oral dose of 2 mg/g in Swiss albino mice.
The findings of the present study may partially support the folkloric use of Capparis tomentosa root for the treatment of pain and inflammation.
山矾科植物山矾的根是埃塞俄比亚 Kunama 族治疗疼痛和炎症的常用传统药物之一。然而，其民族医学的说法尚未得到科学研究。
本研究旨在评估山矾根的 70%乙醇提取物和溶剂部分的抗炎和镇痛活性。
采用体外透明质酸酶抑制试验和体内角叉菜胶诱导的瑞士白化小鼠爪肿胀模型，测定 70%乙醇提取物和溶剂部分的抗炎活性。采用醋酸诱导的扭体模型和瑞士白化小鼠尾浸模型测定镇痛作用。
山矾根提取物具有显著的透明质酸酶抑制活性，与对照药物吲哚美辛相当。与阴性对照组相比，所有测试样品处理的小鼠的角叉菜胶诱导的爪肿胀均得到显著抑制，其中丁醇部分的活性最高，与乙酰水杨酸的作用相当。所有根提取物和溶剂部分均具有显著的、剂量依赖性的腹部扭体抑制作用，其中在水相部分观察到最高的效果。此外，与蒸馏水处理组相比，粗提取物在增加小鼠对热水刺激的反应潜伏期方面表现更好，在 60-120 分钟后，与溶剂部分相比具有显著差异。根提取物和溶剂部分在单口服 2mg/g 的剂量下对瑞士白化小鼠也是安全的。
本研究的结果可能部分支持山矾根用于治疗疼痛和炎症的民间用途。
