Ahmed Zulfiqar, Vohra Muhammad Sufyan, Khan Muhammad Noman, Ahmed Ayaz, Khan Taseer Ahmed
Department of Physiology, University of Karachi, Karachi-75270, Pakistan.
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.
J Infect Dev Ctries. 2019 Feb 28;13(2):130-136. doi: 10.3855/jidc.10542.
The emergence of antimicrobial resistance among bacterial community resulted in a ban on drugs as the growth promoter in poultry feed. This situation demands to explore alternatives as food supplements with health benefit to poultry. Therefore, probiotic microorganisms, which are considered as safe and possess various health benefits can be a choice. Present study was designed to explore the probiotic potential of the isolated lactobacillus species in chickens.
Out of 220 samples, 100 Lactobacillus species were isolated from various regions of chicken intestine. They were further characterized on the basis of morphology, staining and catalase test. Species-level identification was made by amplifying Lactobacillus specific 16S rRNA gene. Out of 100 isolates, 21 were selected for sequencing on the basis of band intensity.
Among 21 sequences, 16 were identified as L. paracasei (n = 6), L. salivarius (n = 3), L. johnsonii (n = 3), and L. agilis, L. fermentum, L. sakei, and L. curvatus (n = 1 each). These strains were found to be significantly acid-tolerant with 81.68 - 85.01% survival rate at pH 2)and bile-tolerant with 81.96 -84.65% survival rate at 0.3% bile. Except three; all strains showed salt tolerance to 2% and 4% NaCl. Among 21 Lactobacillus strains, 6 showed good antimicrobial activities against S. aureus, Salmonella Typhimurium and E. coli.
Lactobacillus species with probiotic property can be used in poultry feed formulation for their health benefit to combat gastrointestinal infections.
细菌群落中抗菌药物耐药性的出现导致家禽饲料中作为生长促进剂的药物被禁用。这种情况需要探索作为对家禽有益健康的食品补充剂的替代品。因此，被认为安全且具有多种健康益处的益生菌微生物可以成为一种选择。本研究旨在探索从鸡中分离出的乳酸杆菌属物种的益生菌潜力。
从220个样本中，从鸡肠道的不同部位分离出100株乳酸杆菌属物种。它们通过形态学、染色和过氧化氢酶试验进一步表征。通过扩增乳酸杆菌属特异性16S rRNA基因进行种水平鉴定。在100株分离物中，根据条带强度选择21株进行测序。
在21个序列中，16个被鉴定为副干酪乳杆菌（n = 6）、唾液乳杆菌（n = 3）、约氏乳杆菌（n = 3），以及敏捷乳杆菌、发酵乳杆菌、清酒乳杆菌和弯曲乳杆菌（各n = 1）。这些菌株被发现具有显著的耐酸性，在pH 2时存活率为81.68 - 85.01%，并且耐胆汁，在0.3%胆汁中存活率为81.96 - 84.65%。除了三株；所有菌株对2%和4% NaCl表现出耐盐性。在21株乳酸杆菌菌株中，6株对金黄色葡萄球菌、鼠伤寒沙门氏菌和大肠杆菌表现出良好的抗菌活性。
具有益生菌特性的乳酸杆菌属物种可用于家禽饲料配方中，因其对对抗胃肠道感染有益健康。
