Gusils C, Pérez Chaia A, González S, Oliver G
Centro de Referencia para Lactobacilos (CERELA), S. M. de Tucumán, Argentina.
J Food Prot. 1999 Mar;62(3):252-6. doi: 10.4315/0362-028x-62.3.252.
Lactobacillus strains were tested for their in vitro probiotic properties. Cell surface hydrophobicity was found to be very high for Lactobacillus fermentum subsp. cellobiosus and Salmonella Gallinarum; high values could indicate a greater ability to adhere to epithelial cells. Studies on Lactobacillus animalis indicated relative cell surface hydrophobicities smaller than those of L. fermentum subsp. cellobiosus and L. fermentum. L. animalis and Enterococcus faecalis were able to coaggregate with L. fermentum subsp. cellobiosus and L. fermentum, respectively, but not with Salmonella Gallinarum. After mixed-culture studies for determining suitable growth behavior, the pair of strains L. animalis plus L. fermentum subsp. cellobiosus was selected for an attempted challenge against Salmonella Gallinarum. Double and triple mixed-culture studies indicated that selected lactobacillus strains were able to retain their beneficial characteristics in the presence of Salmonella Gallinarum such as presence of lectins, production of antimicrobial compounds, and ability to grow and compete. The selected microorganisms can be considered as potential ingredients for a chicken probiotic feed formulation intended to control salmonellosis and also improve poultry sanitation.
对乳酸杆菌菌株进行了体外益生菌特性测试。发现发酵乳杆菌亚种纤维二糖乳杆菌和鸡沙门氏菌的细胞表面疏水性非常高；高数值可能表明其黏附上皮细胞的能力更强。对动物乳杆菌的研究表明，其相对细胞表面疏水性低于发酵乳杆菌亚种纤维二糖乳杆菌和发酵乳杆菌。动物乳杆菌和粪肠球菌能够分别与发酵乳杆菌亚种纤维二糖乳杆菌和发酵乳杆菌共聚集，但不能与鸡沙门氏菌共聚集。在进行混合培养研究以确定合适的生长行为后，选择动物乳杆菌加发酵乳杆菌亚种纤维二糖乳杆菌这对菌株尝试对抗鸡沙门氏菌。双重和三重混合培养研究表明，所选乳酸杆菌菌株在鸡沙门氏菌存在的情况下能够保持其有益特性，如存在凝集素、产生抗菌化合物以及生长和竞争能力。所选微生物可被视为用于控制沙门氏菌病并改善家禽卫生状况的鸡用益生菌饲料配方的潜在成分。
