College of Earth Science and Engineering, Shandong University of Science and Technology, Qingdao, China.
The Second Institute of Hydrogeology and Engineering Geology, Shandong Provincial Bureau of Geology & Mineral Resources, Dezhou, China.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2020;55(5):629-637. doi: 10.1080/10934529.2020.1724744. Epub 2020 Feb 10.
Characterizing bacterial communities is of great significance for targeted control of bacteria-induced clogging during geothermal water recharge. Based on a series of laboratory-scale percolation experiments, the variations in bacterial community diversity, composition, and structure were investigated during simulated geothermal water recharge using high-throughput sequencing technology. The Chao, Shannon, and Evenness indexes were used to quantify the richness, diversity, and evenness of the bacterial community, respectively. The results show that the richness of the bacterial community initially increased and then decreased in the sand columns during the experiments of geothermal water recharge, while the changes in bacterial diversity and evenness were not apparent. A variety of bacterial phyla were found, among which Proteobacteria was predominant (88.31%), followed by Actinobacteria, Bacteroidetes, and Firmicutes (4.23%, 3.44%, and 2.49%). For the non-Proteobacterial phyla, Actinobacteria gradually disappeared while Bacteroidetes and Firmicutes were detected during the percolation experiments. This study implies that, despite the variations in the bacterial community, a core group of bacteria persists during geothermal water recharge, and thus a targeted control of bacteria-induced clogging during geothermal water recharge should be feasible.
描述细菌群落对于有针对性地控制地热回灌过程中由细菌引起的堵塞具有重要意义。本研究基于一系列实验室规模的渗滤实验，利用高通量测序技术研究了模拟地热回灌过程中细菌群落多样性、组成和结构的变化。Chao、Shannon 和 Evenness 指数分别用于量化细菌群落的丰富度、多样性和均匀度。结果表明，在回灌实验中，细菌群落的丰富度在沙柱中先增加后减少，而细菌多样性和均匀度的变化并不明显。发现了多种细菌门，其中变形菌门（Proteobacteria）占主导地位（88.31%），其次为放线菌门（Actinobacteria）、拟杆菌门（Bacteroidetes）和厚壁菌门（Firmicutes）（分别为 4.23%、3.44%和 2.49%）。对于非变形菌门，在渗滤实验中，放线菌逐渐消失，而拟杆菌门和厚壁菌门被检测到。本研究表明，尽管细菌群落发生了变化，但在回灌过程中仍存在一组核心细菌，因此有针对性地控制地热回灌过程中由细菌引起的堵塞是可行的。
