Lange G, Mandelkow E M, Jagla A, Mandelkow E
Max-Planck Unit for Structural Molecular Biology, Hamburg, Federal Republic of Germany.
Eur J Biochem. 1988 Dec 1;178(1):61-9. doi: 10.1111/j.1432-1033.1988.tb14429.x.
Several types of non-equilibrium phenomena have been observed in microtubule polymerization, including dynamic instability, assembly overshoot and oscillations. They can be interpreted in terms of interactions between tubulin subunits (= alpha, beta heterodimers), microtubules, and a third state, oligomers, which represent intermediates between microtubule disassembly and the regeneration of assembly-competent subunits by GTP. Here we examine the role of oligomers by varying conditions that stabilize or destabilize microtubules and/or oligomers. By varying their ratio one can drive tubulin assembly either into steady-state microtubules or oligomers. These regimens of assembly conditions are separated by a region where microtubules oscillate. The oscillations can be simulated by computer modelling, based on a reaction scheme involving the three states of tubulin and nucleotide exchange on tubulin subunits, but not on microtubules or oligomers.
在微管聚合过程中观察到了几种非平衡现象，包括动态不稳定性、组装超调以及振荡。这些现象可以通过微管蛋白亚基（=α、β异二聚体）、微管和第三种状态（寡聚体）之间的相互作用来解释，寡聚体代表了微管解聚与GTP使具有组装能力的亚基再生之间的中间体。在这里，我们通过改变稳定或破坏微管和/或寡聚体的条件来研究寡聚体的作用。通过改变它们的比例，可以将微管蛋白组装驱动到稳态微管或寡聚体中。这些组装条件方案被微管振荡的区域分隔开。基于涉及微管蛋白三种状态以及微管蛋白亚基上核苷酸交换（而非微管或寡聚体上的核苷酸交换）的反应方案，通过计算机建模可以模拟这些振荡。
