Key Laboratory of Textile Science and Technology of Ministry of Education, College of Textiles Donghua University, Shanghai, China.
J Mater Chem B. 2020 Feb 26;8(8):1759-1770. doi: 10.1039/c9tb02522g.
Using Chitosan/PEO as the shell and PCL as the core, chitosan-polyethylene oxide/polycaprolactone nanofibrous mats were prepared successfully by coaxial electrospinning for co-load and sequential co-delivery of two drugs. Herein, lidocaine hydrochloride (Lid), used for pain relief, was added to the shell, and curcumin (Cur), an anti-inflammatory agent, was introduced into the core. Sodium bicarbonate (SB) was also added to the core layer to provide wound microenvironment sensitivity. Under acidic conditions, Lid was released due to the formation of -NH3+ by protonation of -NH2 on the chitosan molecular chains. At the same time, SB reacted with hydrogen ions to generate CO2, and many holes were generated on the surface of the fibers, providing more discharge paths for Cur release. Additionally, both Lid in the shell layer and Cur in the core layer exhibited acidic pH (∼5.4)-responsive release profiles. Moreover, a rapid release of Lid and a sustained release of Cur were observed to provide the immediate effects of analgesia and long-term antibacterial activity in the process of wound healing. Furthermore, after 48 h incubation, the mats showed continuous and excellent antibacterial performance against E. coli and S. aureus. The results of blood coagulation showed that the mats could achieve rapid hemostasis in the early stage of wound formation. Hemolytic and cytotoxicity evaluation also revealed that the mats had excellent hemocompatibility and cytocompatibility. Therefore, this study has made invaluable contributions to the design of a dual-drug-loaded dressing with microenvironment-responsive and sequential release properties towards wound care.
以壳聚糖/PEO 为壳，PCL 为芯，成功地通过同轴静电纺丝制备了壳聚糖-聚乙烯氧化物/聚己内酯纳米纤维垫，用于共载和顺序共递送两种药物。在此，盐酸利多卡因（Lid），用于缓解疼痛，被添加到壳层，姜黄素（Cur），一种抗炎剂，被引入到芯层。同时，在芯层中添加了碳酸氢钠（SB）以提供伤口微环境敏感性。在酸性条件下，由于壳聚糖分子链上的-NH2质子化形成-NH3+，Lid 被释放。同时，SB 与氢离子反应生成 CO2，纤维表面产生许多孔，为 Cur 释放提供了更多的释放途径。此外，壳层中的 Lid 和芯层中的 Cur 均表现出酸性 pH（约 5.4）响应性释放特性。此外，观察到 Lid 的快速释放和 Cur 的持续释放，为伤口愈合过程中的即时镇痛和长期抗菌活性提供了效果。此外，经过 48 h 孵育，垫子对大肠杆菌和金黄色葡萄球菌表现出连续且优异的抗菌性能。凝血实验结果表明，垫子在伤口形成的早期可以实现快速止血。溶血和细胞毒性评价也表明，垫子具有良好的血液相容性和细胞相容性。因此，这项研究为设计具有微环境响应和顺序释放特性的双药物负载敷料在伤口护理方面做出了不可估量的贡献。
