Dos Santos Freire Jamilson, Dos Santos Fernandes Bianca Cristina, da Silva Julia Adelaide Cardeal, da Silva Araújo José Rafael, de Almeida Pedro Marcos, da Costa Júnior Joaquim Soares, da Silva Jurandy Nascimento, de Freitas Sâmya Danielle Lima, Martins Francielle Alline
Programa de Pós-Graduação em Química, Universidade Estadual do Piauí - UESPI , Teresina, Brasil.
Centro de Ciências da Natureza, Universidade Estadual do Piauí - UESPI , Teresina, Brasil.
J Toxicol Environ Health A. 2020 Dec 16;83(23-24):730-747. doi: 10.1080/15287394.2020.1824136. Epub 2020 Sep 30.
has been widely used in folk medicine to treat catarrhal infections, diarrhea, and anemia; however, phytochemical and toxicogenetic data are still lacking. The objective of this study was to examine the phytochemical and antioxidant characteristics as well as assess cytogenotoxicity and antigenotoxicity in hexane (HF), ether (EF) and ethyl acetate (AF) fractions of leaves using bioassay. Phytochemical analysis revealed the presence of saponins and phenolic groups. EF fraction contained a higher content of total phenolics (441.23 ± 1.82 mg GAE/g), while HF fraction showed a higher content of total flavonoids (84.77 ± 5.33 mg QE/g). Higher antioxidant activity was observed in EF (EC 25.06 ± 0.07 µg/ml). Cytotoxic effect was verified for all fractions, but no chromosomal alterations were observed in the assay. With respect to antigenotoxicity, the protective effect of EF and AF fractions was attributed to as evidenced by the modulation of mutagenic action of methyl methanesulfonate (MMS), mainly by inhibiting the development of micronuclei. Among the fractions, EF was considered the most promising, as it exhibited higher antioxidant activity, was not genotoxic, exerted protective activity against the damage induced by MMS and also presented cytotoxic activity, a desired quality in the search for natural anticarcinogenic compounds.
它已在民间医学中广泛用于治疗卡他性感染、腹泻和贫血；然而，植物化学和毒理遗传学数据仍然缺乏。本研究的目的是通过生物测定法研究叶片的己烷（HF）、乙醚（EF）和乙酸乙酯（AF）提取物的植物化学和抗氧化特性，并评估其细胞遗传毒性和抗遗传毒性。植物化学分析表明存在皂苷和酚类基团。EF提取物中总酚含量较高（441.23±1.82毫克没食子酸当量/克），而HF提取物中总黄酮含量较高（84.77±5.33毫克槲皮素当量/克）。在EF中观察到较高的抗氧化活性（EC 25.06±0.07微克/毫升）。所有提取物均证实有细胞毒性作用，但在试验中未观察到染色体改变。关于抗遗传毒性，EF和AF提取物的保护作用归因于甲基磺酸甲酯（MMS）诱变作用的调节，主要是通过抑制微核的形成来证明。在这些提取物中，EF被认为是最有前景的，因为它表现出较高的抗氧化活性，无遗传毒性，对MMS诱导的损伤具有保护活性，并且还具有细胞毒性活性，这是寻找天然抗癌化合物所需的特性。
