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属名. 种名. 一个新的家族成员。

gen. nov., sp. nov. a new member of the family .

机构信息

Department of Microbiology, Faculty of Science, Eötvös Loránd University, Pázmány Péter stny. 1/C, H-1117 Budapest, Hungary.

Department of Plant Anatomy, Faculty of Science, Eötvös Loránd University, Budapest, Pázmány Péter stny. 1/C, H-1117, Hungary.

出版信息

Int J Syst Evol Microbiol. 2020 Mar;70(3):2108-2114. doi: 10.1099/ijsem.0.004025.

Abstract

The Gram-stain-negative, aerobic, non-motile, oxidase- and catalase-positive, rod-shaped yellow-coloured bacterial strain MG-N-17 was isolated from a water sample of Lake Fertő/Neusiedler See (Hungary). Results of phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain forms a distinct linage within the family of the phylum , and its closest relatives are DSM 4136 (94.38 %) and DC2a-G7 (91.55 %). The novel bacterial strain prefers a weak alkaline environment and grows optimally between 22-28 °C in the absence of NaCl. The major isoprenoid quinones are MK-10, MK-11, MK-12 and MK-9. The major cellular fatty acids are anteiso-C, C, Cω5 and iso-C. The polar lipid profile contains phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and four unidentified glycolipids. The assembled draft genome of strain MG-N-17 had 44 contigs with an N50 value 348255 nt, 56.5× genome coverage, total length of 5 910 933 bp and G+C content of 56.9 mol%. Strain MG-N-17 (=DSM 106674=NCAIM B.02643) is proposed as the type strain of a new genus and species in the family , for which the name gen. nov., sp. nov. is proposed.

摘要

革兰氏阴性、需氧、不运动、氧化酶和过氧化氢酶阳性、杆状的黄色细菌菌株 MG-N-17 是从匈牙利的费尔特湖/诺伊西德勒湖的水样中分离出来的。基于 16S rRNA 基因序列的系统发育分析结果表明,该菌株在 门的 科内形成一个独特的谱系,其最接近的亲缘关系是 DSM 4136(94.38%)和 DC2a-G7(91.55%)。新型细菌菌株偏好弱碱性环境,在无 NaCl 的情况下,最佳生长温度为 22-28°C。主要的类异戊二烯醌是 MK-10、MK-11、MK-12 和 MK-9。主要的细胞脂肪酸是 anteiso-C、C、Cω5 和 iso-C。极性脂谱含有磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、两种未鉴定的磷脂和四种未鉴定的糖脂。菌株 MG-N-17 的组装草案基因组有 44 个 contigs,N50 值为 348255 nt,基因组覆盖率为 56.5×,全长为 5910933 bp,GC 含量为 56.9 mol%。菌株 MG-N-17(=DSM 106674=NCAIM B.02643)被提议作为一个新属和种的模式菌株,该属和种被提议命名为 属,新种。

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