Pascual Javier, García-López Marina, González Ignacio, Genilloud Olga
Fundación MEDINA, Avenida del Conocimiento 34, Health Sciences Technology Park, 18016 Granada, Spain.
Present address: Department of Microbial Ecology and Diversity Research, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7 B, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2017 Oct;67(10):3951-3959. doi: 10.1099/ijsem.0.002227. Epub 2017 Sep 14.
A novel chemo-organoheterotrophic bacterium, strain CB-286403, was isolated from a Mediterranean forest soil, collected at Sierra de Tejeda, Almijara and Alhama Natural Park, Spain, by using the Diffusion Sandwich System, a device with 384 miniature diffusion chambers. The 16S rRNA gene sequence analyses identified the isolate as a member of the genus Luteolibacter where the type strains Luteolibacterpohnpeiensis A4T-83 (GenBank acc. no. AB331895), Luteolibacteryonseiensis EBTL01 (JQ319003), Luteolibacterluojiensis DR4-30 (JN630810) and Luteolibacteralgae A5J-41-2 (AB331893) were the closest relatives with similarities of 97.0, 96.3, 96.3 and 94.5 %, respectively. The novel isolate was characterized as a Gram-stain-negative, non-motile, short-rod-shaped bacterium. The strain showed a positive response for catalase and cytochrome-c oxidase, divided by binary fission and/or budding, and exhibited an aerobic metabolism. Strain CB-286403 showed a mesophilic and neutrophilic growth range and showed a nutritional preference for simple sugars and complex protein substrates. Major fatty acids included iso-C14 : 0, C16 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and anteiso-C15 : 0. The predominant respiratory quinone was MK-9. Polar lipids comprised major amounts of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol and minor amounts of three unidentified lipids, a glycolipid, a phospholipid and a phosphoglycolipid. Based on a polyphasic taxonomic characterization, strain CB-286403 represents a novel species of the genus Luteolibacter, for which the name Luteolibacter gellanilyticus sp. nov. is proposed. The type strain is CB-286403 (=DSM 28998=CECT 8659).
利用扩散夹层系统（一种带有384个微型扩散室的装置），从西班牙阿尔米哈拉山脉和阿尔哈马自然公园的塞拉德特赫达采集的地中海森林土壤中分离出一种新型化学有机异养细菌，菌株CB - 286403。16S rRNA基因序列分析确定该分离株为黄杆菌属成员，其中模式菌株波纳佩黄杆菌A4T - 83（GenBank登录号AB331895）、延氏黄杆菌EBTL01（JQ319003）、罗纪黄杆菌DR4 - 30（JN630810）和藻类黄杆菌A5J - 41 - 2（AB331893）是其最相近的亲缘种，相似性分别为97.0%、96.3%、96.3%和94.5%。该新型分离株的特征为革兰氏染色阴性、不运动、短杆状细菌。该菌株过氧化氢酶和细胞色素c氧化酶反应呈阳性，通过二分分裂和/或出芽进行分裂，具有需氧代谢。菌株CB - 286403表现出嗜温和嗜中性的生长范围，对单糖和复合蛋白质底物有营养偏好。主要脂肪酸包括异C14 : 0、C16 : 0、C16 : 1ω7c/异C15 : 0 2 - OH和anteiso - C15 : 0。主要呼吸醌为MK - 9。极性脂质主要包括磷脂酰乙醇胺、磷脂酰甘油和二磷脂酰甘油，还有少量三种未鉴定的脂质、一种糖脂、一种磷脂和一种磷酸糖脂。基于多相分类学特征，菌株CB - 286403代表黄杆菌属的一个新物种，为此提出新名称解结冷胶黄杆菌（Luteolibacter gellanilyticus sp. nov.）。模式菌株为CB - 286403（=DSM 28998=CECT 8659）。
