Ebakota Daniel O, Abiodun Onilude A, Nosa Obayagbona O
Department of Biological Sciences, Microbiology Option, Benson Idahosa University, Benin City, Nigeria.
Department of Microbiology, University of Ibadan, Ibadan, Nigeria.
Food Saf (Tokyo). 2018 Sep 28;6(3):118-125. doi: 10.14252/foodsafetyfscj.2018002. eCollection 2018 Sep.
The Listerial flora and bio-load associated with 411 ready-to-eat (RTE) foods sold at several locations in southern Nigeria was evaluated using phenotypic procedures which included serial dilution and pour plate techniques. Selected strains phenotypically identified from the RTE foods were further identified using virulence gene markers and 16srRNA amplification procedures. All the 90 strains cultured from the RTE foods were subjected to antibiogram analysis using disc diffusion. Some of the antibiotics employed included; Ceftazidine, cefuroxime, gentamicin, ofloxacin, augumentin, tetracycline and erythromycin. and were detected in the RTE foods. Haemolysin () gene, Internalin gene () and invasive gene () were detected in all isolates. LMEW70 with accession number KY053295 was 93% similar to L1846. All the isolates were resistant to amoxicillin, cloxacillin, augumentin and ceftazidime.
采用包括系列稀释和平板倾注技术在内的表型方法，对在尼日利亚南部多个地点销售的411种即食（RTE）食品中的李斯特菌菌群和生物负荷进行了评估。从即食食品中表型鉴定出的选定菌株，进一步使用毒力基因标记和16srRNA扩增程序进行鉴定。对从即食食品中培养出的所有90株菌株进行了纸片扩散法药敏分析。使用的一些抗生素包括：头孢他啶、头孢呋辛、庆大霉素、氧氟沙星、奥格门汀、四环素和红霉素。并且在即食食品中被检测到。在所有分离株中均检测到溶血素（）基因、内化素基因（）和侵袭基因（）。登录号为KY053295的LMEW70与L1846的相似度为93%。所有分离株均对阿莫西林、氯唑西林、奥格门汀和头孢他啶耐药。
