Taneja Vani, Nekkanti Sridhar, Gupta Kanishk, Hassija Jyoti
Department of Pedodontics & Preventive Dentistry, Manipal College of Dental Sciences (MCODS), Manipal, Karnataka, India.
J Int Soc Prev Community Dent. 2019 Nov 4;9(6):576-583. doi: 10.4103/jispcd.JISPCD_265_19. eCollection 2019 Nov-Dec.
This study aimed to investigate the remineralization potential of two concentrations of theobromine (100 mg/L and 200 mmg/L) with fluoridated dentifrice, NovaMin, and nanohydroxyapatite using DIAGNOdent, scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) analysis.
Two sections were taken from 50 teeth each. Artificial carious lesions were induced using demineralizing solution. Evaluation using DIAGNOdent, SEM, and EDX analysis for elemental evaluation of Ca/P ratio and fluoride ion was carried out. Teeth sections were then randomly assigned to five different groups: (1) fluoridated dentifrice (Colgate™, Colgate -Palmolive, India), Novamine- Shy NM™, Group pharamaceuticals, India), 3. Nano-hydroxyapatite- Remin Pro™, Voco, Germany) 4. 100mg and 5. 200mg of Theobromine toothpaste (Theodent classic™, Rennou, UK-853069003006). Remineralization was carried out for 14 days with two applications per day. Samples were reanalyzed using DIAGNOdent, SEM, and EDX.
A Tukey test revealed statistically significant difference between NovaMin and all the other toothpastes ( < 0.001) for DIAGNOdent readings. On performing SEM-EDX analysis, it was seen that all agents had remineralization potential; however, no significant difference was found.
Theobromine can be used as an effective novel remineralizing agent alternative to the already-available agents.
本研究旨在使用DIAGNOdent、扫描电子显微镜（SEM）和能量色散X射线（EDX）分析，研究两种浓度的可可碱（100毫克/升和200毫克/升）与含氟牙膏、诺瓦敏和纳米羟基磷灰石的再矿化潜力。
从50颗牙齿中各取两片。使用脱矿溶液诱导人工龋损。使用DIAGNOdent、SEM和EDX分析进行评估，以对钙/磷比和氟离子进行元素评估。然后将牙齿切片随机分为五组：（1）含氟牙膏（高露洁™，高露洁-棕榄公司，印度），（2）诺瓦敏-Shy NM™，印度集团制药公司），（3）纳米羟基磷灰石-Remin Pro™，德国VOCO公司），（4）100毫克和（5）200毫克可可碱牙膏（Theodent classic™，英国Rennou公司-853069003006）。每天进行两次应用，进行14天的再矿化。使用DIAGNOdent、SEM和EDX对样品进行重新分析。
Tukey检验显示，对于DIAGNOdent读数，诺瓦敏与所有其他牙膏之间存在统计学显著差异（<0.001）。在进行SEM-EDX分析时，可以看出所有试剂都有再矿化潜力；然而，未发现显著差异。
可可碱可作为一种有效的新型再矿化剂，替代现有的试剂。
