Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Aging (Albany NY). 2020 Feb 10;12(3):2747-2763. doi: 10.18632/aging.102774.
Cancer immunotherapy has achieved positive clinical responses in the treatment of various cancers, including gastric cancer (GC). In this study, we characterized the heterogeneity of T cells isolated from GC patients at the single-cell level using single-cell RNA sequencing. We identified different immune cell subtypes and their heterogeneous transcription factors and depicted their developmental trajectories. In particular, we focused on exhausted CD8 cells and Tregs and discovered that, as compared to control, the IRF8 transcription factor was downregulated in CD8 tumour-infiltrating lymphocytes (TILs) from GC tissues, and that GC patients with lower IRF8 levels in blood CD8 T cells tended to be a at a more advanced disease stage. These findings provide a theoretical basis for targeted immune therapy in GC.
癌症免疫疗法在治疗各种癌症方面取得了积极的临床反应，包括胃癌（GC）。在这项研究中，我们使用单细胞 RNA 测序在单细胞水平上对从 GC 患者中分离出的 T 细胞的异质性进行了表征。我们鉴定了不同的免疫细胞亚型及其异质性转录因子，并描绘了它们的发育轨迹。特别是，我们关注耗尽的 CD8 细胞和 Tregs，并发现与对照相比，GC 组织中 CD8 肿瘤浸润淋巴细胞（TIL）中的 IRF8 转录因子下调，而血液 CD8 T 细胞中 IRF8 水平较低的 GC 患者往往处于更晚期的疾病阶段。这些发现为 GC 的靶向免疫治疗提供了理论基础。
